Intraspecifična karakterizacija izolata Vibrio alginolyticus prikupljenih iz sustava akvakulture i morskih biotopa u Tunisu korištenjem PCR-RFLP, ECP i OMPS profiliranja

IF 1 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Acta Adriatica Pub Date : 2023-01-01 DOI:10.32582/aa.64.2.3

Rim Lajnef, L. Ben Abdallah, S. Tapia‐Paniagua, Yosra Turki, A. Medina, I. Mehri, M. Moriñigo, A. Hassen

{"title":"Intraspecifična karakterizacija izolata Vibrio alginolyticus prikupljenih iz sustava akvakulture i morskih biotopa u Tunisu korištenjem PCR-RFLP, ECP i OMPS profiliranja","authors":"Rim Lajnef, L. Ben Abdallah, S. Tapia‐Paniagua, Yosra Turki, A. Medina, I. Mehri, M. Moriñigo, A. Hassen","doi":"10.32582/aa.64.2.3","DOIUrl":null,"url":null,"abstract":"A total of 54 presumptive Vibrio alginolyticus strains isolated from a variety of Tunisian marine biotopes, such as seawater, sediment samples from some bathing areas, and aquaculture farms, were characterized and identified by several biochemical tests. DNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, extracellular products (ECP) and outer membrane protein (OMP) profiling were used to evaluate their usefulness as a tool to investigate the Vibrio alginolyticus diversity within this complex group. Results showed that there is great heterogeneity in the diversity observed via the PCR-RFLP method related to the number of genotypes generated by the two enzymes SduI and FaqI tested. This heterogeneity was observed not only according to the origin (seawater, sediment, fish and bivalve aquaculture farms) but also within the same type of sample. The two other methods, ECP and OMP resulted in 32 and 26 profiles, respectively. The discriminatory index determined in this study highlighted the good ability of the PCR-RFLP method to discriminate similar V. alginolyticus strains.","PeriodicalId":35248,"journal":{"name":"Acta Adriatica","volume":"1 1","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Adriatica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.32582/aa.64.2.3","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

A total of 54 presumptive Vibrio alginolyticus strains isolated from a variety of Tunisian marine biotopes, such as seawater, sediment samples from some bathing areas, and aquaculture farms, were characterized and identified by several biochemical tests. DNA polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, extracellular products (ECP) and outer membrane protein (OMP) profiling were used to evaluate their usefulness as a tool to investigate the Vibrio alginolyticus diversity within this complex group. Results showed that there is great heterogeneity in the diversity observed via the PCR-RFLP method related to the number of genotypes generated by the two enzymes SduI and FaqI tested. This heterogeneity was observed not only according to the origin (seawater, sediment, fish and bivalve aquaculture farms) but also within the same type of sample. The two other methods, ECP and OMP resulted in 32 and 26 profiles, respectively. The discriminatory index determined in this study highlighted the good ability of the PCR-RFLP method to discriminate similar V. alginolyticus strains.

查看原文本刊更多论文

Intrspecična karakterizacija izolata溶藻弧菌prikupljenih iz sustava akvakulture i morskih biotopa u Tunisu korištenjem PCR-RFLP，ECP i OMPS profiliranja

从突尼斯各种海洋生物群落(如海水、一些浴场的沉积物样本和水产养殖场)中分离出的54株疑似溶藻弧菌菌株通过若干生化试验进行了特征鉴定和鉴定。利用DNA聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析、细胞外产物(ECP)和外膜蛋白(OMP)分析来评估它们作为研究该复杂群体内溶藻弧菌多样性的工具的有效性。结果表明，通过PCR-RFLP方法观察到的多样性与所测两种酶SduI和FaqI产生的基因型数量存在较大的异质性。这种异质性不仅在不同的来源(海水、沉积物、鱼类和双壳类养殖场)之间存在，而且在同一类型的样本中也存在。另外两种方法，ECP和OMP分别得到32个和26个剖面。本研究测定的鉴别指数表明，PCR-RFLP方法对相似的溶藻弧菌具有良好的鉴别能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Acta Adriatica 生物-海洋与淡水生物学

CiteScore

1.60

自引率

11.10%

发文量

审稿时长

>12 weeks

期刊介绍： Journal "Acta Adriatica" is an Open Access journal. Users are allowed to read, download, copy, redistribute, print, search and link to material, and alter, transform, or build upon the material, or use them for any other lawful purpose as long as they attribute the source in an appropriate manner according to the CC BY licence.