Molecular Profiling and Optimization Studies for PHB Production in Rhodobacter sphaeroides

IF 0.5 Q4 ENERGY & FUELS
Y. Lee, Sangmin Lee
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Abstract

In the recent climate change regime, industrial demand for renewable materials to replace petroleum-derived polymers continues to rise. Of particular interest is polyhydroxybutyrate (PHB) as a substitute for polypropylene. Accumulating evidence indicates that PHB is highly produced as a carbon storage material in various microorganisms. The effects of growth conditions on PHB production have been widely studied in chemolithotrophs, particularly in Rhodobacter . However, the results on PHB production in Rhodobacter have been somewhat inconsistent due to different strains and experimental conditions, and it is currently unclear how diverse environmental factors are linked with PHB production. Here, we report optimized growth conditions for PHB production and show that the growth conditions are closely related to reactive oxygen species (ROS) regulation. PHB accumulates in cells up to approximately 50% at the highest level under dark-aerobic conditions as opposed to light aerobic/anaerobic conditions. According to the time-course, PHB contents increased at 48 h and then gradually decreased. When observing the effect of temperature and medium composition on PHB production, 30 °C and a carbon/nitrogen ratio of 9:1 or more were found to be most effective. Among PHB biosynthetic genes, PhaA and PhaB are highly correlated with PHB production, whereas PhaC and PhaZ showed little change in overall expression levels. We found that, while the amount of hydrogen peroxide in cells under dark conditions was relatively low compared to the light conditions, peroxidase activities and expression levels of antioxidant-related genes were high. These observations suggest optimal culture conditions for growth and PHB production and the importance of ROS-scavenging signaling with regard to PHB production.
球形红杆菌产PHB的分子谱分析及优化研究
在最近的气候变化情况下,工业对可再生材料取代石油衍生聚合物的需求持续上升。特别令人感兴趣的是聚羟基丁酸酯(PHB)作为聚丙烯的替代品。越来越多的证据表明,PHB作为碳储存材料在各种微生物中大量产生。在趋化养石菌中,生长条件对PHB产生的影响已被广泛研究,特别是在红杆菌中。然而,由于菌株和实验条件的不同,在Rhodobacter中产生PHB的结果有些不一致,目前还不清楚不同的环境因素如何与PHB的产生联系。在这里,我们报告了PHB生产的优化生长条件,并表明生长条件与活性氧(ROS)调节密切相关。在暗好氧条件下,PHB在细胞中的蓄积量高达约50%,与光好氧/厌氧条件相反。PHB含量在48 h时呈先上升后下降的趋势。当观察温度和介质组成对PHB产量的影响时,发现30°C和碳氮比为9:1或更高是最有效的。在PHB生物合成基因中,PhaA和PhaB与PHB的产生高度相关,而PhaC和PhaZ的总体表达水平变化不大。我们发现,与光照条件相比,黑暗条件下细胞中过氧化氢的含量相对较低,但过氧化物酶活性和抗氧化相关基因的表达水平较高。这些观察结果提示了生长和PHB产生的最佳培养条件,以及ros清除信号对PHB产生的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advances in Energy Research
Advances in Energy Research ENERGY & FUELS-
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