Development of Theoretical Approaches to Determination of the Main Groups of Biologically Active Substances of Medicinal Plant Raw Materials by TLC Method

Q3 Pharmacology, Toxicology and Pharmaceutics
O. V. Trineeva
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引用次数: 4

Abstract

Introduction. As is known, the selection of optimal conditions for the analysis of extracts from medicinal plant raw materials (MPМ), as well as medicinal herbal preparations and pharmaceutical substances of plant origin, characterized by a complex variable composition of biologically active substances (BAS) by TLC, presents certain difficulties. For the design of mobile phases for the separation of mixtures of BAS of plant origin, in a thin layer of sorbent, the following approaches are used: literary sources; standard mobile phases; spot elution method; the scheme proposed by the firm Camag (Switzerland); model "PRISMA"; variocameras and others. In foreign literature, there are publications on the generalization of the available experimental data on the determination of various natural groups of BAS in objects of plant origin. However, such reviews did not reveal the regularities of the chromatographic behavior of individual BAS in a thin layer, as well as the influence of various factors on the reproducibility of Rf values. The study of the possibility of a theoretical approach to the choice of optimal conditions for chromatography of groups of BAS of different polarity, allowing them to separate, identify and quantify by TLC is a relevant and poorly developed area of chromatography in general.Aim. The aim of this work was to develop a theoretical approach to the choice of optimal conditions for the chromatographic separation of various groups of BAS of plant origin in a thin layer of sorbent.Materials and methods. To study the regularities of chromatographic behavior in a thin layer of representatives of the main classes of BAS present in MPМ (amino acids, flavonoids, tannins, simple sugars, ascorbic acid, fat-soluble vitamins), the value of the main factor affecting the parameters of the efficiency of the chromatographic process, the polarity of the eluent, was studied. As objects of research, we used ready-made chopped raw material of nettle leaves, produced by a domestic manufacturer, that meets the requirements of regulatory documents, as well as sea buckthorn fruits collected on the territory of the Voronezh region, according to the rules for harvesting MPМ of various morphological groups in fresh and dried form.Results and discussion. The regularities of elution and mathematical models describing the chromatographic behavior of plant BAS in a thin layer of sorbent have been established. Based on the totality of the results obtained, from the standpoint of the efficiency of the chromatographic process, the optimal conditions for their TLC analysis were selected and theoretically substantiated. To study the qualitative composition of BAS and to achieve a clear separation of zones on chromatograms, TLC methods were developed and tested on the studied MPМ using simple, frontal or two-dimensional chromatography.Conclusion. It is shown that the determination and separation in a thin layer of the sorbent of hydrophilic and lipophilic BAS of MPМ in the presence of a joint requires different approaches and techniques. The paper proposes an algorithm for the selection of the mobile phase and methods of chromatography of BAS of medicinal products. The revealed mathematical models describing the chromatographic behavior of BAS will make it possible to select the conditions under which it is possible to determine the individual components of multicomponent mixtures without preliminary separation. The developed methods for the determination of BAS can also be used for standardization and quality assessment of other types of MPМ, phytopreparations and pharmaceutical substances of plant origin.
薄层色谱法测定药用植物原料药生物活性物质主要类群理论方法的建立
介绍。众所周知,药用植物原料提取物(MPМ)以及具有复杂多变生物活性物质(BAS)组成特征的草药制剂和植物源性原料药的TLC分析,其最佳条件的选择存在一定的困难。对于分离植物源BAS混合物的流动相的设计,在一层薄薄的吸附剂中,使用以下方法:文献资料;标准流动相;斑点洗脱法;由Camag公司(瑞士)提出的方案;模型“棱镜”;可变相机和其他。在国外文献中,有出版物对现有的实验数据进行了概括,以确定植物源对象中各种天然类群的BAS。然而,这些综述并没有揭示单个BAS在薄层中的色谱行为规律,以及各种因素对Rf值重现性的影响。研究一种理论方法的可能性,以选择不同极性的BAS群的最佳色谱条件,使它们能够通过TLC进行分离、鉴定和定量,这是色谱学中一个相关但欠发达的领域。这项工作的目的是发展一种理论方法,以选择最佳条件的色谱分离的各种组的植物源的BAS薄层吸附剂。材料和方法。为了研究MPМ中存在的主要BAS类(氨基酸、黄酮类、单宁类、单糖、抗坏血酸、脂溶性维生素)代表的薄层色谱行为的规律,研究了影响色谱过程效率的主要因素参数的取值,即洗脱液的极性。作为研究对象,我们使用了国内制造商生产的符合监管文件要求的现成切碎的荨麻叶原料,以及在沃罗涅日地区收集的沙棘果实,根据收获规则MPМ以新鲜和干燥的形式收获各种形态组。结果和讨论。建立了植物BAS在薄层吸附剂中的洗脱规律和描述其色谱行为的数学模型。根据所得结果,从色谱过程效率的角度出发,选择了其TLC分析的最佳条件,并从理论上进行了验证。为了研究BAS的定性组成,并在色谱上实现清晰的区域分离,建立了TLC方法,并在研究的MPМ上使用简单、正面或二维色谱进行了测试。结果表明,在亲水性和亲脂性BAS (MPМ)吸附剂的薄层中,在有关节存在的情况下,需要不同的方法和技术来测定和分离。本文提出了一种流动相选择算法和药品BAS色谱分析方法。所揭示的描述BAS色谱行为的数学模型将使选择不需要预先分离就可以确定多组分混合物中单个组分的条件成为可能。建立的BAS测定方法也可用于其它类型MPМ、植物修复制剂和植物源性原料药的标准化和质量评价。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Drug Development and Registration
Drug Development and Registration Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
1.20
自引率
0.00%
发文量
61
审稿时长
8 weeks
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