Genomic properties in Achromobacter spp. strains from cystic fibrosis patients in Russia

Q4 Medicine

Klinicheskaia mikrobiologiia i antimikrobnaia khimioterapiia Pub Date : 2021-01-01 DOI:10.36488/cmac.2021.3.220-225

Y. Bocharova, T.A. Saviniova, A. V. Chaplin, A. Lyamin, O. Kondratenko, S. Polikarpova, S. Zhilina, N. Fedorova, M. Korzhanova, N. A. Mayansky, I. Chebotar

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引用次数: 0

Abstract

Objective. To determine species, sequence-types, antimicrobial resistance and virulence genes in Achromobacter spp. isolates obtained from cystic fibrosis (CF) patients in Russia. Materials and Methods. Samples (sputum, nasopharyngeal swab) from 168 CF patients from 48 regions were studied. Whole-genome sequencing (WGS) was performed on MGISEQ-2000 platform. SPAdes software, Galaxy, ResFinder, Integrall, PubMLST were used for analysis of WGS data. Results. A total of 18 strains of Achromobacter spp. were isolated from 16 of 168 CF patients.Achromobacter xylosoxidans was the most prevalent and detected in 13⁄18 cases (72%). Studied Achromobacter spp. isolates belonged to 14 sequence types, including 8 new sequence types. An adaptive resistance gene carriage was a rare phenomenon (1⁄18 isolates). Conclusions. The Achromobacter spp. colonization rate of respiratory system in CF patients in Russia corresponds to the data reported in other countries. A. xylosoxidans isolates were the most prevalent (72%). Achromobacter spp. isolates from CF patients in Russia and show a high clonal diversity.

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俄罗斯囊性纤维化患者无色杆菌菌株的基因组特性

目标。目的:测定俄罗斯囊性纤维化(CF)患者分离的无色杆菌菌株的种类、序列类型、耐药性和毒力基因。材料与方法。研究了来自48个地区168例CF患者的痰、鼻咽拭子样本。全基因组测序(WGS)在MGISEQ-2000平台上进行。采用SPAdes、Galaxy、ResFinder、Integrall、PubMLST软件对WGS数据进行分析。结果168例CF患者中16例共分离到18株无色杆菌。其中以氧化木糖无色杆菌最常见，检出率为13 / 18(72%)。所分离的无色杆菌共有14种序列型，其中8种为新序列型。携带适应性耐药基因是一种罕见的现象(1 / 18株)。俄罗斯CF患者呼吸系统Achromobacter spp.定殖率与国外报道数据一致。以木糖氧化单胞菌最常见(72%)。从俄罗斯CF患者中分离的无色杆菌显示出较高的克隆多样性。

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来源期刊

Klinicheskaia mikrobiologiia i antimikrobnaia khimioterapiia Medicine-Epidemiology

CiteScore

0.90

自引率

0.00%

发文量

审稿时长

8 weeks