The phenomenon of root elongation and high respiration activity in the rolB-gene-enhanced FSL#35 variant of Lotus corniculatus FOX-SR line

IF 1 Q3 PLANT SCIENCES

Plant Root Pub Date : 2014-01-01 DOI:10.3117/PLANTROOT.8.82

Tsubasa Yano, A. Yamamoto, H. Kunitake, Y. Saeki, R. Akashi

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引用次数: 2

Abstract

For systematic functional analysis of genes, we attempted the application of the FOX-hunting system to super growing-roots (SR) of legume species Lotus corniculatus which was previously reported by Himuro et al. (2011). In this study, we investigated the functional analysis of FSL#35, which was expressed by the rolB gene derived from the Agrobacterium rhizogenes Ri plasmid. In monoculture roots grown in liquid media, the FSL#35 showed specific phenotypes that increased root length, lateral root number and root surface area compared with SR. These enhanced phenotypes of FSL#35 were caused by cell profile alteration, while increased total root length and increased lateral root number were caused by the expansion of cortex cells and increased pericycle cells, respectively. In addition, the FSL#35 root showed high and specific respiration activity compared with SR. These results suggest that distinct cell profiles of FSL#35 were induced by the alteration of respiration activity in root tissue. The enhanced root growth in the FSL#35 root might be induced by alteration of ROS metabolisms. Investigating the details of the rolB gene function, for example by phytohormone analysis, will elucidate the novel benefits of the rolB gene for agriculture.

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rolb基因增强莲藕FOX-SR系FSL#35变异的根伸长和高呼吸活性现象

为了对基因进行系统的功能分析，我们尝试将FOX-hunting系统应用于Himuro et al.(2011)先前报道的豆科植物莲花(Lotus corniculatus)的超生长根(SR)。本研究对根状农杆菌Ri质粒中rolB基因表达的FSL#35进行了功能分析。在液体培养基中培养的单根中，FSL#35表现出比sr更高的根长、侧根数和根表面积的特殊表型，这些表型的增加是由细胞形态改变引起的，而总根长和侧根数的增加分别是由皮质细胞和中柱鞘细胞的扩增引起的。此外，与sr相比，FSL#35的根系呼吸活性表现出较高的特异性。这些结果表明，FSL#35不同的细胞形态是由根系呼吸活性的改变引起的。FSL#35根系生长增强可能与活性氧代谢改变有关。研究rolB基因功能的细节，例如通过植物激素分析，将阐明rolB基因对农业的新益处。

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来源期刊

Plant Root PLANT SCIENCES-

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.