Genetic analyses of anthocyanin content using polyploid GWAS followed by QTL detection in the sweetpotato (Ipomoea batatas L.) storage root

IF 1 Q3 PLANT SCIENCES
Plant Root Pub Date : 2020-01-01 DOI:10.3117/plantroot.14.11
Emdadul M. Haque, E. Yamamoto, K. Shirasawa, Hiroaki Tabuchi, U. Yoon, S. Isobe, Masaru Tanaka
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引用次数: 6

Abstract

: Genetic studies on the purple-fleshed sweetpotato ( Ipomoea batatas L.), which is rich in anthocyanin (AN) in the storage root, were performed by polyploid GWAS based on the allele dosage probability using 59,675 SNPs obtained from 94 F 1 progenies between the cultivars ‘Konaishin’ (which has a high yield but no AN) and ‘Akemurasaki’ (which has a high AN content but low to moderate yield). The distribution of relative AN content was highly biased, with 60% of clones showing a low to undetectable level (A 530 < 0.5). Fifty-nine SNPs from six signals on homologous groups (HGs) 3, 5 (one major and one smaller sig-nal), 7, 13, and 15 were strongly associated with the relative AN content. Twelve SNPs from the major signal and one from the smaller signal of HG 5 were further detected by QTL analysis. In a database search of the AN biosynthesis gene, transcription factors IbMYB1 and IbWD40 and AN structural genes IbF3H and IbDFR were located on HG 5, suggesting that an SNP marker or markers from HG 5 might be tightly linked to candidate gene(s) homologous to one of these transcription factors and AN structural genes as a major factor in determining AN accumulation in the storage roots. These results would enhance our understanding of the underlying genetic basis of AN accumulation in the storage roots of sweetpotatoes, and the SNP markers found here, especially 13 SNPs from HG 5, would be a potential platform for future marker-assisted selection for breeding high-AN sweetpotato varieties.
甘薯贮藏根花青素含量的多倍体GWAS - QTL遗传分析
利用高产不含AN的“Konaishin”和高产不含AN的“Akemurasaki”94个F - 1后代的59,675个单核苷酸多态性,采用多倍体GWAS方法对贮藏根中花青素含量较高的紫肉甘薯(Ipomoea batatas L.)进行了等位基因剂量概率遗传研究。相对AN含量的分布高度偏倚,60%的克隆显示低至不可检测水平(a530 < 0.5)。同源基团(HGs) 3、5(一个大信号和一个小信号)、7、13和15的6个信号中的59个snp与相对AN含量密切相关。通过QTL分析进一步检测到来自HG 5主信号的12个snp和来自较小信号的1个snp。在对AN生物合成基因的数据库搜索中,转录因子IbMYB1和IbWD40以及AN结构基因IbF3H和IbDFR位于HG 5上,这表明HG 5上的一个或多个SNP标记可能与这些转录因子和AN结构基因同源的候选基因紧密相连,是决定AN在贮藏根中积累的主要因素。这些结果将加深我们对甘薯贮藏根中AN积累的潜在遗传基础的理解,并且该研究发现的SNP标记,特别是来自HG 5的13个SNP标记,将为未来标记辅助选择高AN甘薯品种提供潜在的平台。
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来源期刊
Plant Root
Plant Root PLANT SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
期刊介绍: Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.
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