Dose-dependent effects of simvastatin, atorvastatin and rosuvastatin on apoptosis and inflammation pathways on cancerous lung cells

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Ankara Universitesi Veteriner Fakultesi Dergisi Pub Date : 2022-01-27 DOI:10.33988/auvfd.938418

Nursel Dikmen, H. Özkan, Funda Cimen, B. Camdeviren, E. Ay, P. Ambarcioglu, N. Duran, Akın Yakin

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引用次数: 0

Abstract

The aim of study was to investigate the anti-proliferative and inflammatory effects of atorvastatin, rosuvastatin, and simvastatin in lung cancer. The effects of statins were investigated in Vero, BEAS-2B, and A549 cell lines. In addition to expressions of BAX, BCL-2, TNFα, IL-10, IL-6 , protein levels of TNFα, IL-10, IL-6 were determined. Cell viability and MDA were also measured. While the cell numbers in groups with low doses of statins were found to be approximately 1x10 6 /mL, proliferation was inhibited at higher rates containing high doses. Simvastatin, rosuvastatin, and high dose atorvastatin upregulated the BAX , while high dose of atorvastatin and both doses of rosuvastatin caused downregulation in BCL-2 . All statin groups had higher MDA. Simvastatin and high dose rosuvastatin upregulated TNFα . While low dose simvastatin and atorvastatin and high dose atorvastatin and rosuvastatin upregulated IL-10 , IL-6 was upregulated with a low dose of rosuvastatin. TNFα was higher in simvastatin and rosuvastatin groups. IL-10 was highest in rosuvastatin groups. Atorvastatin groups had lower IL-6. Although cell numbers have been reduced by all statins, rosuvastatin is more effective on studied genes.

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辛伐他汀、阿托伐他汀和瑞舒伐他汀对肺癌细胞凋亡和炎症通路的剂量依赖性影响

本研究的目的是探讨阿托伐他汀、瑞舒伐他汀和辛伐他汀在肺癌中的抗增殖和炎症作用。研究了他汀类药物在Vero、BEAS-2B和A549细胞系中的作用。除BAX、BCL-2、TNFα、IL-10、IL-6表达外，检测TNFα、IL-10、IL-6蛋白表达水平。同时测定细胞活力和MDA。低剂量他汀类药物组的细胞数量约为1 × 10 6 /mL，高剂量他汀类药物组的增殖抑制率更高。辛伐他汀、瑞舒伐他汀和高剂量阿托伐他汀可上调BAX，而高剂量阿托伐他汀和两种剂量瑞舒伐他汀均可下调BCL-2。所有他汀类药物组均有较高的MDA。辛伐他汀和大剂量瑞舒伐他汀上调TNFα。低剂量辛伐他汀和阿托伐他汀以及高剂量阿托伐他汀和瑞舒伐他汀上调IL-10，而低剂量瑞舒伐他汀上调IL-6。辛伐他汀组和瑞舒伐他汀组TNFα升高。瑞舒伐他汀组IL-10最高。阿托伐他汀组IL-6较低。虽然所有他汀类药物都能减少细胞数量，但瑞舒伐他汀对所研究的基因更有效。

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来源期刊

Ankara Universitesi Veteriner Fakultesi Dergisi 农林科学-兽医学

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ankara Üniversitesi Veteriner Fakültesi Dergisi is one of the journals’ of Ankara University, which is the first well-established university in the Republic of Turkey. Research articles, short communications, case reports, letter to editor and invited review articles are published on all aspects of veterinary medicine and animal science. The journal is published on a quarterly since 1954 and indexing in Science Citation Index-Expanded (SCI-Exp) since April 2007.