Seperation of bioactive compounds from Haemolymph of scarab beetle Scarabaeus sacer (Coleoptera: Scarabaeidae) by GC-MS and determination of its antimicrobial activity

International journal of applied biology and pharmaceutical technology Pub Date : 2021-01-01 DOI:10.26502/ijabpt.202113

Nancy Taha Mohamed, Doaa Hassan Abdelsalam, Ahmed Salem El-Ebiarie, M. Elaasser

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引用次数: 2

Abstract

This study aimed to investigate the bioactive compounds in the haemolymph of scarab beetle Scarabaeus sacer by using the Gas chromatography–mass spectrometry (GS-MS) analysis. The identification of the bioactive compounds is based on peak area, retention time, molecular formula and molecular weight. There are 129 compounds are detected in the haemolymph of scarab beetle and 43 of them were reported to have a bioactivity. The most analyzed bioactive compounds are alcohols, steroids, fatty acids and terpenoids. The current study also test the antimicrobial activity of scarab beetle haemolymph against gram-negative bacteria (Escherichia coli, Enterobacter cloacae, grampositive bacteria (Staphylococcus aureus, Bacillus subtilis), and fungi (Aspergillus fumigatus and Candida albicans). The haemolymph has highest antibacterial activity against gram negative bacteria Enterobacter cloacae, Int J Appl Biol Pharm 2021; 12 (4): 461-480 DOI: 10.26502/ijabpt.202113 International Journal of Applied Biology and Pharmaceutical Technology Vol. 12 No. 4 December 2021. 462 Escherichia coli respectively and against gram-positive bacteria Bacillus subtilis, Staphylococcus aureus respectively. No antifungal activity has been detected.

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用气相色谱-质谱法分离金龟甲(鞘翅目:金龟甲科)血淋巴中活性物质及其抑菌活性

本研究采用气相色谱-质谱联用技术(gc - ms)对圣甲虫血淋巴中的生物活性物质进行了研究。生物活性化合物的鉴定是基于峰面积、保留时间、分子式和分子量。在圣甲虫的血淋巴中检测到129种化合物，其中43种具有生物活性。分析最多的生物活性化合物是醇类、类固醇、脂肪酸和萜类。目前的研究还测试了圣甲虫血淋巴对革兰氏阴性细菌(大肠杆菌、阴沟肠杆菌)、革兰氏阳性细菌(金黄色葡萄球菌、枯草芽孢杆菌)和真菌(烟曲霉和白色念珠菌)的抗菌活性。血淋巴对革兰氏阴性细菌阴沟肠杆菌的抗菌活性最高，Int J apple Biol Pharm 2021;12 (4): 461-480 DOI: 10.26502/ijabpt.202113国际应用生物学与制药技术杂志Vol. 12 No. 4 December 2021。分别抗革兰氏阳性菌枯草芽孢杆菌、金黄色葡萄球菌。未检测到抗真菌活性。

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International journal of applied biology and pharmaceutical technology

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