Bacterial Species Identified in the Filtrate of Microfiltration Membranes in the Separation of Activated Sludge

Q3 Environmental Science

Journal of Water and Environment Technology Pub Date : 2021-01-01 DOI:10.2965/jwet.21-086

Shuai Zhou, Manae Ninoseki, Asahi Kusaba, Kazuma Nakagawa, T. Urase

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引用次数: 1

Abstract

Membrane bioreactor-reverse osmosis process (MBR-RO) is widely applied in wastewater treatment, especially when high-quality treated water is needed. Although MBR shows better performances in the removal of pathogens than the conventional activated sludge process, stable operation of RO process in the latter step of MBR-RO depends on residual bacteria in the filtrate of microfiltration membranes. Species and sizes of bacteria found in the filtrate of activated sludge with 0.2 μm pore-size polycarbonate membranes were investigated in this study. Isolated bacterial species grown on R2A agar medium were identified based on the full length 16S rRNA gene sequences. The result showed that approximately 90% of the isolates found in the filtrates were members of phylum Pro teobacteria in which Ralstonia spp., Achromobacter spp., Methylobacterium spp. and Methylorubrum spp. accounted for the largest proportions while other bacteria affiliated with phyla Actinobacteria and Firmicutes. The leakage of Ralstonia and Actinobacteria was probably due to their small sizes judging from microscopic observation, while a longer filtration time is needed for Methylobacterium and Methylorubrum to be detected in the filtrate.

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活性污泥分离中微滤膜滤液中细菌种类的鉴定

膜生物反应器-反渗透工艺(MBR-RO)在污水处理中得到了广泛的应用，特别是在需要高质量处理水的情况下。虽然MBR在去除病原体方面表现出比传统活性污泥工艺更好的性能，但MBR-RO后一阶段RO工艺的稳定运行取决于微滤膜滤液中的残留细菌。研究了孔径为0.2 μm聚碳酸酯膜的活性污泥滤液中细菌的种类和大小。根据16S rRNA基因的全长序列，在R2A琼脂培养基上鉴定分离菌种。结果表明，滤液中约90%的分离菌属于原生细菌门，其中Ralstonia spp、Achromobacter spp、Methylobacterium spp.和Methylorubrum spp.所占比例最大，其他细菌隶属于放线菌门和厚壁菌门。从显微镜观察来看，Ralstonia和Actinobacteria的渗漏可能是由于其体积较小，而Methylobacterium和Methylorubrum则需要较长的过滤时间才能在滤液中检测到。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Water and Environment Technology Environmental Science-Water Science and Technology

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

43 weeks

期刊介绍： The Journal of Water and Environment Technology is an Open Access, fully peer-reviewed international journal for all aspects of the science, technology and management of water and the environment. The journal’s articles are clearly placed in a broader context to be relevant and interesting to our global audience of researchers, engineers, water technologists, and policy makers. JWET is the official journal of the Japan Society on Water Environment (JSWE) published in English, and welcomes submissions that take basic, applied or modeling approaches to the interesting issues facing the field. Topics can include, but are not limited to: water environment, soil and groundwater, drinking water, biological treatment, physicochemical treatment, sludge and solid waste, toxicity, public health and risk assessment, test and analytical methods, environmental education and other issues. JWET also welcomes seminal studies that help lay the foundations for future research in the field. JWET is committed to an ethical, fair and rapid peer-review process. It is published six times per year. It has two article types: Original Articles and Review Articles.