Evaluation of C. perfringens cpe-positive Strain as a Source Tracking Indicator of Human Contamination in Freshwater Environments

Q3 Environmental Science

Journal of Water and Environment Technology Pub Date : 2021-01-01 DOI:10.2965/JWET.20-089

Hiroyuki Suzuki, K. Oonaka, A. Hashimoto

{"title":"Evaluation of C. perfringens cpe-positive Strain as a Source Tracking Indicator of Human Contamination in Freshwater Environments","authors":"Hiroyuki Suzuki, K. Oonaka, A. Hashimoto","doi":"10.2965/JWET.20-089","DOIUrl":null,"url":null,"abstract":"In this study, a field survey of fecal contamination indicators and Clostridium perfringens cpe(+) in river environments was conducted to verify the effectiveness of C. perfringens cpe(+) as a microbial source tracking indicator in public water bodies. In the Saijo River, which serves as a model of point source contamination from human sewage effluents, the concentrations of Escherichia coli, enterococci and C. perfringens increased after the inflow of sewage effluents, with the cpe-positive prevalence rate of C. perfringens isolates increasing from 13.3 to 21.5%. In the Nagaike River, contaminated with household sewage effluents from non-point sources, high concentrations of E. coli, enterococci, and C. perfringens cpe(+) were detected, with high cpe-positive prevalence rates of 27.6 and 26.4%. Contrarily, in the Koayu River, which is contaminated with treated wastewater from a large swine farm, an increase in the concentration of fecal contamination indicators and C. perfringens was observed; however, the cpe-positive prevalence rate of C. perfringens isolates remained unchanged, ranging as low as 3.6 and 3.9%. Altogether, our results revealed that the concentration and pollution load of C. perfringens cpe(+) is an effective microbial source tracking indicator of human fecal contamination in freshwater environments.","PeriodicalId":17480,"journal":{"name":"Journal of Water and Environment Technology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Water and Environment Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2965/JWET.20-089","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Environmental Science","Score":null,"Total":0}

引用次数: 2

Abstract

In this study, a field survey of fecal contamination indicators and Clostridium perfringens cpe(+) in river environments was conducted to verify the effectiveness of C. perfringens cpe(+) as a microbial source tracking indicator in public water bodies. In the Saijo River, which serves as a model of point source contamination from human sewage effluents, the concentrations of Escherichia coli, enterococci and C. perfringens increased after the inflow of sewage effluents, with the cpe-positive prevalence rate of C. perfringens isolates increasing from 13.3 to 21.5%. In the Nagaike River, contaminated with household sewage effluents from non-point sources, high concentrations of E. coli, enterococci, and C. perfringens cpe(+) were detected, with high cpe-positive prevalence rates of 27.6 and 26.4%. Contrarily, in the Koayu River, which is contaminated with treated wastewater from a large swine farm, an increase in the concentration of fecal contamination indicators and C. perfringens was observed; however, the cpe-positive prevalence rate of C. perfringens isolates remained unchanged, ranging as low as 3.6 and 3.9%. Altogether, our results revealed that the concentration and pollution load of C. perfringens cpe(+) is an effective microbial source tracking indicator of human fecal contamination in freshwater environments.

查看原文本刊更多论文

产气荚膜荚膜梭菌cpe阳性菌株作为淡水环境人类污染源跟踪指标的评价

本研究通过对河流环境中粪便污染指标和产气荚膜梭菌cpe(+)的实地调查，验证了产气荚膜梭菌cpe(+)作为公共水体微生物源跟踪指标的有效性。作为人类生活污水点源污染模型的西条河，污水入流后大肠杆菌、肠球菌和产气荚膜荚膜梭菌浓度升高，产气荚膜荚膜梭菌cpe阳性流行率从13.3%上升至21.5%。在被非点源生活污水污染的永池河中，检测到高浓度的大肠杆菌、肠球菌和产气荚膜杆菌cpe(+)， cpe阳性阳性率分别为27.6%和26.4%。相反，在被大型养猪场处理过的废水污染的Koayu河，观察到粪便污染指标和产气荚膜梭菌的浓度增加;然而，产气荚膜荚膜荚膜原分离株的cpe阳性患病率保持不变，低至3.6%和3.9%。综上所述，产气荚膜荚膜梭菌cpe(+)的浓度和污染负荷是淡水环境中人类粪便污染的有效微生物源跟踪指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Water and Environment Technology Environmental Science-Water Science and Technology

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

43 weeks

期刊介绍： The Journal of Water and Environment Technology is an Open Access, fully peer-reviewed international journal for all aspects of the science, technology and management of water and the environment. The journal’s articles are clearly placed in a broader context to be relevant and interesting to our global audience of researchers, engineers, water technologists, and policy makers. JWET is the official journal of the Japan Society on Water Environment (JSWE) published in English, and welcomes submissions that take basic, applied or modeling approaches to the interesting issues facing the field. Topics can include, but are not limited to: water environment, soil and groundwater, drinking water, biological treatment, physicochemical treatment, sludge and solid waste, toxicity, public health and risk assessment, test and analytical methods, environmental education and other issues. JWET also welcomes seminal studies that help lay the foundations for future research in the field. JWET is committed to an ethical, fair and rapid peer-review process. It is published six times per year. It has two article types: Original Articles and Review Articles.