Biosynthesis of cyclotides

4区生物学 Q2 Agricultural and Biological Sciences

Advances in Botanical Research Pub Date : 2015-01-01 DOI:10.26181/5E3A3D90E6328

Thomas M A Shafee, K. Harris, M. Anderson

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引用次数: 9

Abstract

Book chapter postprint DOI: 10.1016/bs.abr.2015.08.005 License: Sherpa/Romeo 0065-2296 Abstract: Cyclotides are gene-encoded, ribosomally produced cyclic proteins. Their biosynthesis is a multistep process involving several enzymes for translation, disulphide isomerization, transport, cleavage and, finally, cyclization. Here, we describe this process, focusing on the cyclization step and the vacuolar processing enzyme (VPE) that performs it. Cyclotide precursor proteins contain prodomains that direct organelle targeting and contain sequences necessary for cyclization in addition to the one or more domains that make up the final cyclic product. The VPEs are a family of cysteine proteases that perform cyclization by an adaptation of their more commonly described proteolysis mechanism. VPE-mediated cyclization is dependent on the presence of short recognition sequences (within and flanking the mature cyclotide domain) which have been characterized in vivo and in vitro for the prototypical cyclotide kalata B1. Finally, we compare cyclization by VPEs to other methods of protein cyclization found in nature or developed for biotechnology. Understanding cyclotide biosynthesis is important for both fundamental science, and for broader protein cyclization applications.

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环聚糖的生物合成

摘要:环核苷酸是由基因编码、核糖体产生的环状蛋白。它们的生物合成是一个多步骤的过程，包括翻译、二硫异构化、运输、裂解和最后的环化。在这里，我们描述了这个过程，重点是环化步骤和执行它的液泡处理酶(VPE)。环肽前体蛋白含有指导细胞器靶向的原结构域，除了组成最终环产物的一个或多个结构域外，还含有环化所需的序列。vpe是一个半胱氨酸蛋白酶家族，通过适应其更常见的蛋白质水解机制来进行环化。vpe介导的环化依赖于短识别序列的存在(在成熟环肽结构域内和侧翼)，这些序列已经在体内和体外对原型环肽kalata B1进行了表征。最后，我们将vpe的环化与其他在自然界发现的或为生物技术开发的软蛋白环化方法进行了比较。了解环核苷酸的生物合成对基础科学和更广泛的蛋白质环化应用都很重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in Botanical Research 生物-植物科学

CiteScore

4.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Advances in Botanical Research (including Advances in Plant Pathology) have been published since 1963. The series has surpassed 85 volumes and features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This includes a comprehensive series of volumes dealing with the genome evolution of plants. Recent developments on plant metabolomics have also been described.