Immunohistochemical analysis of selected stem cell markers including stage-specific mouse embryonic antigen 1 (SSEA-1) in porcine and bovine endometrium throughout the oestrous cycle

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria Brno Pub Date : 2023-01-01 DOI:10.2754/avb202392030251

P. Konecná, Dominika Macháčová, F. Tichý, L. Fiala, M. Kyllar, J. Lenz

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Abstract

There has been a lack of research regarding endometrial stem cells in farm animals, and particularly, regarding epithelial stem/progenitor cells. We aimed to identify potential stem cell subpopulations in porcine and bovine endometrium by measuring the expression of selected stem cell markers (OCT3/4, CD44, SSEA-1, SOX10, CD73, and CD117) during the follicular and luteal phases of the oestrous cycle. We studied 28 endometrial tissue samples collected from 14 healthy, cycling pigs and cows. The endometrial mucosa of each sample was divided into basal, middle, and luminal portions, and the immunohistochemical staining intensity and percentages of cells that were marker-positive were recorded for each portion of the endometrium. Expression of OCT-3/4 was identified in the glands and stroma, and that of SOX10 and CD117 was identified in stroma of both porcine and bovine endometrium. In porcine endometrium, CD44 was only expressed in the glands, whereas SSEA-1 was expressed in the glands and stroma. In bovine endometrium, CD73 was only expressed in the glands. Differences in the expression of most of these markers were found between phases of the oestrous cycle and/or portions of the endometrial mucosa. Our data indicate the existence of both epithelial and mesenchymal stem cell subpopulations in the endometrium of pigs and cows during their oestrous cycles. The expression pattern of the stem cell marker SSEA-1 in porcine endometrium suggests a possible alternative location of the epithelial stem/progenitor cell population in the middle portion of the endometrial glands.

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在整个发情周期中，猪和牛子宫内膜中包括阶段特异性小鼠胚胎抗原1 (SSEA-1)在内的选定干细胞标记物的免疫组织化学分析

关于农场动物子宫内膜干细胞，特别是上皮干细胞/祖细胞的研究一直缺乏。我们旨在通过测量在发情周期卵泡期和黄体期选定的干细胞标记物(OCT3/4、CD44、SSEA-1、SOX10、CD73和CD117)的表达，确定猪和牛子宫内膜中潜在的干细胞亚群。我们研究了从14头健康的骑自行车的猪和牛身上采集的28个子宫内膜组织样本。将每个样本的子宫内膜粘膜分为基底、中、腔部分，记录各部分子宫内膜免疫组化染色强度和标记阳性细胞百分比。OCT-3/4在腺和间质中均有表达，SOX10和CD117在猪和牛子宫内膜间质中均有表达。在猪子宫内膜中，CD44仅在腺体中表达，而SSEA-1在腺体和间质中表达。在牛子宫内膜中，CD73仅在腺体中表达。在发情周期的不同阶段和/或部分子宫内膜粘膜中发现了大多数这些标记物的表达差异。我们的数据表明，在猪和奶牛的发情周期中，子宫内膜中存在上皮细胞和间充质干细胞亚群。干细胞标记物SSEA-1在猪子宫内膜中的表达模式表明，上皮干细胞/祖细胞群可能位于子宫内膜腺体的中间部分。

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来源期刊

Acta Veterinaria Brno 农林科学-兽医学

CiteScore

1.00

自引率

33.30%

发文量

审稿时长

18-36 weeks

期刊介绍： ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic. The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.