The effects of egg yolk-based and egg yolk-free diluents on the post-thaw quality of bull spermatozoa

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria Brno Pub Date : 2022-01-01 DOI:10.2754/avb202291040339

J. Vodička, J. Pytlík, M. Stádníková, L. Stádník, J. Ducháček, R. Codl, Zuzana Biniová

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引用次数: 2

Abstract

The aim of this study was to evaluate the effects of 3 different commercially available extenders – AndroMed® (soy lecithin-based), Optidyl® (with the addition of ionized egg yolk), and BULLXcell® (with the addition of fresh egg yolk) – on bull spermatozoa quality, which was evaluated using the parameters of spermatozoa motility, viability, plasma membrane damage, acrosome damage, and mitochondrial activity after thawing and during a 2 h long thermoresistance test. The spermatozoa quality indicators were appraised by computer-assisted semen analysis and a flow cytometer. Significant differences (P < 0.01) between bulls were registered in all indicators measured. The highest average values of spermatozoa total motility and viability were achieved using BULLXcell® extender (44.33%; 52.06%). Variances in comparing this extender with Optidyl® and AndroMed® were −0.83%, −2.64%; −8.33%, −9.51%. The differences found between the egg yolk-based diluents (BULLXcell® and Optidyl®) and AndroMed® were significant (P < 0.01). Therefore, the more valuable extenders for bull semen dilution were egg yolk-based extenders, which provided higher post-thaw spermatozoa survival and quality than the soy lecithin-based extender.

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蛋黄基和不含蛋黄的稀释剂对公牛精子解冻后品质的影响

本研究的目的是评估3种不同的市售扩展剂——AndroMed®(大豆卵磷脂为基础)、Optidyl®(添加电离蛋黄)和BULLXcell®(添加新鲜蛋黄)对公牛精子质量的影响，通过精子活力、活力、质膜损伤、顶体损伤和线粒体活性等参数来评估解冻后和2小时的抗热性测试。用计算机辅助精液分析和流式细胞仪评价精子质量指标。各指标在公牛间均有极显著差异(P < 0.01)。使用BULLXcell®扩展剂精子总活力和活力的平均值最高(44.33%;52.06%)。该扩展剂与Optidyl®和AndroMed®的差异分别为- 0.83%和- 2.64%;−−8.33%,9.51%。蛋黄基稀释液(BULLXcell®和Optidyl®)与AndroMed®之间差异显著(P < 0.01)。因此，以蛋黄为基础的公牛精液稀释剂比以大豆卵磷脂为基础的公牛精液稀释剂具有更高的解冻后精子存活率和质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Veterinaria Brno 农林科学-兽医学

CiteScore

1.00

自引率

33.30%

发文量

审稿时长

18-36 weeks

期刊介绍： ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic. The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.