A Molecular survey of Hepatozoon canis in dogs in the Siirt province of Turkey

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria Brno Pub Date : 2022-01-01 DOI:10.2754/avb202291030277

B. Çelik, Ö. Çelik, Adnan Ayan, A. Yılmaz, Ö. Kilinç, Ö. Ayan

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引用次数: 0

Abstract

This study aimed to determine Hepatozoon canis prevalence in dogs in the Siirt province of Turkey by the molecular method. The animal material of the study consisted of a total of 75 dogs that appeared clinically healthy. Two ml of blood sample were taken from the vena cephalica antebrachii. Then, DNA extraction was performed. Polymerase chain reaction (PCR) was performed to amplify the 666 bp 18S rRNA gene region of Hepatozoon canis. Two positive PCR products were purified and sequenced. As a result of Nested-PCR, H. canis specific bands in 666 bp size were obtained in 7 (9.33%) out of 75 dogs. The result of sequence analysis, the nucleotide sequence was registered in the NCBI GenBank database with accession numbers OL467380.1-OL467538.1. Hepatozoon canis registered in GenBank of sequence OL467380.1 was found to be similar with other H. canis strains of registration numbers MW684292.1 with 99.69% and MH615006.1-MK091085.1-MF797806.1 with 99.53% rates; and the sequence with registration number OL467538.1 was found to be similar to the series MW684291.1 with 99.09% and MH615006.1-MK091085.1-KX 818220.1 with 99.08% rates by BLAST analysis. Hepatozoon canis prevalence of dogs in the Siirt province was determined as a result of this study. It is of great importance to take preventive measures, especially to fight ticks with appropriate acaricides, since there is no vaccine to prevent the disease.

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土耳其锡尔特省犬类犬肝虫的分子调查

本研究旨在通过分子方法确定土耳其锡尔特省犬中犬肝虫的流行情况。该研究的动物材料包括75只临床表现健康的狗。从头前静脉取血2 ml。然后进行DNA提取。采用聚合酶链反应(PCR)扩增了犬肝虫666 bp的18S rRNA基因区。对两个阳性PCR产物进行纯化和测序。巢式pcr结果显示，75只狗中有7只(9.33%)获得666 bp大小的犬嗜血杆菌特异性条带。序列分析结果，核苷酸序列已在NCBI GenBank数据库中注册，登录号为ol467380.1 ~ ol467538.1。在GenBank序列OL467380.1中登记的犬肝动物与登记号为MW684292.1的犬肝动物相似率为99.69%，与登记号为MH615006.1-MK091085.1-MF797806.1的犬肝动物相似率为99.53%;经BLAST分析，与MH615006.1-MK091085.1-KX 818220.1序列的相似性为99.08%，与登记号为OL467538.1的序列相似性为99.09%。本研究确定了锡尔特省犬类犬肝虫病流行率。采取预防措施是非常重要的，特别是使用适当的杀螨剂来对抗蜱虫，因为没有疫苗来预防这种疾病。

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来源期刊

Acta Veterinaria Brno 农林科学-兽医学

CiteScore

1.00

自引率

33.30%

发文量

审稿时长

18-36 weeks

期刊介绍： ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic. The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.