ВЛИЯНИЕ ДОФАМИНА ПЛАЗМЫ НА УРОВЕНЬ АЛЬФА-СИНУКЛЕИНА Ш45+-КЛЕТОК КРОВИ ПРИ БОЛЕЗНИ ПАРКИНСОНА

Ucenye Zapiski SanktPeterburgskogo Gosudarstvennogo Medicinskogo Universiteta im Akad IP Pavlova Pub Date : 2015-06-30 DOI:10.24884/1607-4181-2015-22-2-14-17

П. А. Андоскин, А. К. Емельянов, Михаил Алексеевич Николаев, К. А. Сенкевич, В. П. Шилин, А. А. Тимофеева, А. Ф. Якимовский, Софья Николаевна Пчелина

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引用次数: 1

Abstract

Metabolic impairment of alpha-synuclein protein is considered to be the central event in PDpathogenesis. Recent studies explored usage of alpha-synuclein in peripheral fluids as a biomarker of PD, however alpha-synuclein level in the CSF and plasma is considered to be affected by hemolysis. In order to avoid contamination of a lymphocyte fraction by erythrocytes, we have proposed an algorithm based on measurements of alpha-synuclein levels in the homogeneous CD45+ cell blood fraction. For this study we formed a group of PD patients (N=14) and a control group without the neurological disorders (N=17). We found an increase in the level of the total alpha-synuclein in CD45+ cells of PD patients compared to controls (p = 0,04), and revealed a direct correlation between the level of dopamine in plasma and level of total alpha-synuclein in CD45+ cells in the control group (r=0,71, p = 0,007).The level of alpha-synuclein in CD45+ cells could be suggested as possible PD biomarker.

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多巴胺对帕金森氏症患者s45 +血细胞水平的影响

α -突触核蛋白的代谢损伤被认为是pd发病的中心事件。最近的研究探索了外周液体中α -突触核蛋白作为PD的生物标志物的使用，然而脑脊液和血浆中的α -突触核蛋白水平被认为受到溶血的影响。为了避免红细胞对淋巴细胞部分的污染，我们提出了一种基于测量均质CD45+细胞血液部分α -突触核蛋白水平的算法。在本研究中，我们分为PD患者组(N=14)和无神经系统疾病的对照组(N=17)。我们发现PD患者CD45+细胞中总α -synuclein水平较对照组增加(p = 0,04)，并揭示了对照组血浆多巴胺水平与CD45+细胞中总α -synuclein水平之间的直接相关性(r=0,71, p = 0,007)。α -突触核蛋白在CD45+细胞中的表达水平可能作为PD的生物标志物。

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Ucenye Zapiski SanktPeterburgskogo Gosudarstvennogo Medicinskogo Universiteta im Akad IP Pavlova

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8 weeks