The mature and immature spermatozoa of the free-living marine nematode Daptonema sp. (Nematoda: Monhysterida: Xyalidae)

Abstract

The immature spermatozoa stored in the testis and female postvulval uterine sac (PUS), and mature spermatozoa from the uterus of the free-living marine nematode Daptonema sp. (Monhysterida: Monhysterina: Xyalidae) were studied. The spermatozoa have a nucleus without a nuclear envelope. The central cytoplasm of the immature spermatozoa from the testis and the distal part of the PUS is occupied by a mass of pale fibrous bodies (FB) surrounded by mitochondria and osmiophilic membranous organelles (MO). The spermatozoa in the proximal half of PUS have a wide peripheral layer of electron lucent filamentous cytoplasm. The uterus lumen contains an aggregation of mature spermatozoa of which the periphery is transformed into pseudopods. The FB replaced by a voluminous electron-lucent halo bounded by a continuous layer of MO, mitochondria and fibrous matter. The MO may be intact, but numerous MO are fused with the plasma membrane, having been transformed into transparent pouches, each one opening to the exterior via a pore. Ultrastructural data showed that the activation is regulated in the female when spermatozoa migrate toward the uterus and transform into amoeboid mature spermatozoa. In general, the spermatozoa of Daptonema sp. and some other Monhysterina closely resemble those of the taxa belonging to the order Rhabditida. However, the `rhabditid' pattern of spermatozoon structure and development is most likely the plesiomorphic state in Rhabditida and close sister groups.