APPLICATION DNA FINGERPRINT ANALYSIS FOR IDENTIFICATION OF MIXED GROUPS OF SIBERIAN STURGEON (ACIPENSER BAERI BRANDT)

Abstract

DNA finferprinting analysis based on microsatellites was applied for separation of mixed gynogenetic offspring of Siberian sturgeon (Acipenser baeri) and individuals from commercial production. Variation at 11 microsatellite DNA loci was surveyed for parent of gynogenetic offspring. Thus microsatellite DNA profiles in studied loci were known and this key-point was aplied in segregation analysis of mixed fish. In results 108 individuals of 281 studied were verified as gynogenetic offspring. The present survey of microsatellite variation demonstrated a reliable tool for separation of mixed group of fish.