Different tobacco cultivation regions lead to variations in tobacco leaf gene expression profiles involved in carbonhydrate metabolism and ion transportation

Q3 Agricultural and Biological Sciences
Liming Lu, Wen-yu Xu, Yong Chen, Zong-xiang Tang, Lei Liu, Liqin Li
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引用次数: 0

Abstract

Tobacco is an important crop economically in China, and tobacco quality varies across its cultivated regions. To unveil the mechanisms that result in such varied tobacco leaf qualities, we have analyzed the tobacco leaf gene expression profiles of four areas in Sichuan province, China. Tobacco leaves in the 12 position were collected from plants in four different regions, namely Huili, Miyi, Xingwen and Jiange, and submitted for microarray analysis. Gene expression levels from Miyi group were used as control, and that from Jiange, Xingwen and Huili were compared to Miyi group respectively. A total of 5154 differentially expressed genes (DEGs) were detected, among which 2731 genes were up-regulated and 2423 genes were down-regulated. Based on gene ontology (GO) analysis, significant DEGs were classified into 15 categories (P≤0.05) based on properties pertaining to transferase activity, transmembrane transporter activity, binding, transcription regulator activity, metabolic processes, secondary metabolic processes, etc. Our results also show that these DEGs were significantly enriched in 11 pathways, including metabolic pathways, starch and sucrose metabolism, photosynthesis, etc. Our study suggests that many of the genes involved in various plant physiological and biochemical processes significantly differed across these four different regions, which may explain their differences in tobacco leaf quality.
烟叶碳水化合物代谢和离子转运相关基因的表达谱在不同的种植区域存在差异
烟草是中国重要的经济作物,不同种植地区的烟草质量参差不齐。为了揭示导致烟叶品质差异的机制,我们分析了中国四川省四个地区的烟叶基因表达谱。从会理、米邑、兴文、江阁4个不同地区的植株上采集12个位置的烟叶,进行基因芯片分析。以米易组的基因表达量为对照,分别与米易组的江阁、兴文和会理基因表达量进行比较。共检测到5154个差异表达基因(DEGs),其中上调2731个,下调2423个。基于基因本体(GO)分析,根据转移酶活性、跨膜转运蛋白活性、结合、转录调节剂活性、代谢过程、次生代谢过程等特性,将显著性deg分为15类(P≤0.05)。我们的研究结果还表明,这些deg在11个途径中显著富集,包括代谢途径、淀粉和蔗糖代谢、光合作用等。我们的研究表明,在这四个不同的区域,参与植物各种生理生化过程的许多基因存在显著差异,这可能解释了它们在烟叶品质上的差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Plant Omics
Plant Omics 生物-植物科学
CiteScore
1.30
自引率
0.00%
发文量
0
审稿时长
6 months
期刊介绍: Plant OMICS is an international, peer-reviewed publication that gathers and disseminates fundamental and applied knowledge in almost all area of molecular plant and animal biology, particularly OMICS-es including: Coverage extends to the most corners of plant and animal biology, including molecular biology, genetics, functional and non-functional molecular breeding and physiology, developmental biology, and new technologies such as vaccines. This journal also covers the combination of many areas of molecular plant and animal biology. Plant Omics is also exteremely interested in molecular aspects of stress biology in plants and animals, including molecular physiology.
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