IDENTIFICATION OF GENES UP-REGULATED IN RESPONSE TO LEAD EXPOSURE IN SUNFLOWER (Helianthus annuus L.)

M. Osman, A. El-Shanshoury, M. Amer, M. Faheem, R. Sammour
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引用次数: 1

Abstract

In this study, Differential display reverse transcription (DDRT) technique was used to analyze differentially upregulated genes in Helianthus annuus in response to Pb exposure. Seeds of sunflower were germinated in lead contaminated soils and untreated plants were used as control. Twenty differentially expressed fragments were identified and characterized. The fragments were classified according to their expression levels. The significance of the function of the identified differentially expressed genes was discussed in relation to their possible roles as stress genes. Two fragments showed no significant homology with any database sequences in the GenBank. Results of the database sequence alignment identified two fragments showed homology to unclassified and unknown genes; two cDNA fragments show homology with salinity responsive genes, three fragments showed homology with drought responsive genes, one fragment showed homology with cold responsive gene, one fragment showed homology with oxidative responsive gene and one fragment showed homology with biotic stress resistance gene. More importantly, a fragment had significant homology with EST of Glycine max similar to serine/threonine-protein kinase gene, a fragment showed significant homology with EST of Arabidopsis thaliana putative anion transporter 2 (ANTR2) mRNA, a fragment had significant homology with EST of Helianthus annuus delta-12 oleate desaturase (FAD2- 2) gene, a fragment showed significant homology with EST of Dianthus caryophyllus putative MtN3-like protein mRNA, two fragments showed significant homology with EST for NADH dehydrogenase chain 3 (nad3) genes, a fragment had significant homology with EST from Volvox sp. similar to cytochrome oxidase subunit 1(CoxI) and a fragment showed significant homology with EST23 of Medicago truncatula MLP-like protein. These results implicate that several pathways are involved in the plant's response to Pb exposure which still needs to be elucidated further.
向日葵(Helianthus annuus L.)铅暴露上调基因的鉴定
本研究采用差分显示反转录(DDRT)技术分析了向日葵(Helianthus annuus)对Pb暴露的差异上调基因。向日葵种子在铅污染土壤中萌发,以未经处理的植物为对照。鉴定鉴定了20个差异表达片段。根据其表达水平对片段进行分类。讨论了所鉴定的差异表达基因的功能意义及其作为应激基因的可能作用。两个片段与GenBank数据库序列无明显同源性。数据库序列比对结果显示,两个片段与未分类和未知基因同源;2条cDNA片段与盐度响应基因同源,3条片段与干旱响应基因同源,1条片段与寒冷响应基因同源,1条片段与氧化响应基因同源,1条片段与生物抗性基因同源。更重要的是,一个片段与甘氨酸max丝氨酸/苏氨酸蛋白激酶基因的EST具有显著的同源性,一个片段与拟南芥推测阴离子转运蛋白2 (ANTR2) mRNA的EST具有显著的同源性,一个片段与向日葵δ -12油酸去饱和酶(FAD2- 2)基因的EST具有显著的同源性,一个片段与石竹推测mtn3样蛋白mRNA的EST具有显著的同源性。NADH脱氢酶链3 (nad3)基因的两个片段与EST具有显著的同源性,一个片段与Volvox sp.的EST具有显著的同源性,类似于细胞色素氧化酶亚基1(CoxI),一个片段与Medicago truncatula mlp样蛋白的EST23具有显著的同源性。这些结果表明,植物对铅暴露的反应涉及多种途径,这些途径仍需进一步阐明。
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