CHARACTERIZATION OF SOME RICE GENOTYPES FOR FER-TILITY RESTORING GENES USING RAPD AND SSR

Abstract

With the objective of identifying restorers and maintainers, to identify fertility restoration linked markers that can distinguish cytoplasmic sources and restorer lines, four male sterile and fertile counterparts of Cytoplasmic Male Sterile (CMS) lines and 10 Restorers (R) lines were characterized. Based on spikelet fertility percentages, ten suggested restorer were identified. Giza 182, Giza 178 and Giza 181 were identified as restorers for the CMS lines G46A and D297A. Ten SSR and 12 RAPD markers were used for the identification of the fertility restoration gene in restorer lines. Among ten suggested restorer lines, only three Egyptian rice cultivars, Giza 182, Giza 178 and Giza 181 used in molecular analysis. Two out of ten SSR markers (RM493 and RM510) could differentiate the CMS line, restorer and their hybrids. RM493 produced bands with size of 123, 128 and 126 bp in the restorer cultivars Giza 181, Giza 182 and Giza 178, respectively. Whereas, the SSR marker RM510 produced bands with size of 165, 186 and 162 bp in the restorer cultivars Giza 181, Giza 182 and Giza 178, respectively. These specific markers were found in their corresponding hybrids with CMS line G46A. In addition, two RAPD primers OPK08 and OPK10 produced fertility restoration linked markers with the molecular size of 200 and 690 bp, respectively. These markers were found only in the restorer cultivars and their respective hybrids with the CMS line G46A. The results suggested effective utilization of SSR and RAPD markers in hybrid testing and marker aided heterosis breeding in rice.