ISOLATION AND CHARACTERIZATION OF ACROSIN GENE (Acr), EXON 5 IN THE BUCKS OF GOAT BREEDS

Abstract

Three Egyptian (Baladi, Barki and Zaraibi) and one Syrian (Damascus) goat breeds were used to isolate and characterize acrosin gene, exon 5. A sample of ten bucks individuals from Zaraibi and Damascus breeds and five bucks individuals from Baladi and Barki breeds were randomly taken and DNA was extracted from each individual and analyzed with the acrosin specific primers. One band with a fragment size of a 378 bp in all individual samples for all breeds represent exon 5 of Acr gene was obtained. Sequence analysis showed that Baladi and Zaraibi breeds were identical and differ from sequences of Damascus and Barki breeds (who also identical) by one base pair number 266 (T in Baladi and Zaraibi breeds while it was C in Barki and Damascus breeds). The genetic analysis for Acr gene, exon 5 showed that this region was conserved among the studied goat breeds.