Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations

IF 1 4区 工程技术 Q4 CHEMISTRY, APPLIED
M. Radonjic, J. Petrovic, M. Milivojevic, M. Stevanović, Jasmina Stojkovska, B. Obradovic
{"title":"Chemical engineering methods in analyses of 3D cancer cell cultures: Hydrodinamic and mass transport considerations","authors":"M. Radonjic, J. Petrovic, M. Milivojevic, M. Stevanović, Jasmina Stojkovska, B. Obradovic","doi":"10.2298/ciceq210607033r","DOIUrl":null,"url":null,"abstract":"A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors, while chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 mm s-1 induced lower viability of NT2/D1 cells in superficial microbead zones implying adverse effects of fluid shear stresses estimated as ~67 mPa. On the contrary, similar velocity (100 mm s-1) enhanced proliferation of C6 glioma cells within microfibers as compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that medium partially flows through the hydrogel (interstitial velocity of ~10 nm s-1). Thus, a diffusion-advection-reaction model was applied to describe the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of ?10-9-10-11 m2 s-1 are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (?10-19 m2 s-1) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.","PeriodicalId":9716,"journal":{"name":"Chemical Industry & Chemical Engineering Quarterly","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Industry & Chemical Engineering Quarterly","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.2298/ciceq210607033r","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 1

Abstract

A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors, while chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 mm s-1 induced lower viability of NT2/D1 cells in superficial microbead zones implying adverse effects of fluid shear stresses estimated as ~67 mPa. On the contrary, similar velocity (100 mm s-1) enhanced proliferation of C6 glioma cells within microfibers as compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that medium partially flows through the hydrogel (interstitial velocity of ~10 nm s-1). Thus, a diffusion-advection-reaction model was applied to describe the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of ?10-9-10-11 m2 s-1 are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (?10-19 m2 s-1) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.
化学工程方法在分析三维癌细胞培养:流体力学和质量运输的考虑
基于实验和数学建模的多学科方法被用于癌细胞三维(3D)培养的仿生系统开发。具体而言,将人胚胎畸胎癌NT2/D1和大鼠胶质瘤C6两种癌细胞分别固定在海藻酸微珠和微纤维中,在灌注生物反应器中静态和流动条件下培养,并应用化学工程方法解释所得结果。表面介质流速为80 mm s-1时,表面微珠区NT2/D1细胞活力降低,表明流体剪切应力的不利影响约为~67 mPa。相反,与静态对照相比,相似的速度(100 mm s-1)增强了微纤维内C6胶质瘤细胞的增殖。另外对银/蜂蜜/海藻酸盐纳米复合微纤维在灌注下的银释放进行了研究,发现介质部分流过水凝胶(间隙速度为~10 nm s-1)。因此,采用扩散-平流-反应模型来描述物质在微纤维内向固定细胞的转移。扩散系数为- 10-9-10-11 m2 s-1的物质仅由扩散供给,而扩散系数明显较低的物质(?10-19 m2 s-1)需要额外的对流输送。本研究展示了化学工程方法在肿瘤模型系统开发中的选择和贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Chemical Industry & Chemical Engineering Quarterly
Chemical Industry & Chemical Engineering Quarterly CHEMISTRY, APPLIED-ENGINEERING, CHEMICAL
CiteScore
2.10
自引率
0.00%
发文量
24
审稿时长
3.3 months
期刊介绍: The Journal invites contributions to the following two main areas: • Applied Chemistry dealing with the application of basic chemical sciences to industry • Chemical Engineering dealing with the chemical and biochemical conversion of raw materials into different products as well as the design and operation of plants and equipment. The Journal welcomes contributions focused on: Chemical and Biochemical Engineering [...] Process Systems Engineering[...] Environmental Chemical and Process Engineering[...] Materials Synthesis and Processing[...] Food and Bioproducts Processing[...] Process Technology[...]
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信