Optimization of megakaryocyte trapping for platelet formation in microchannels

IF 1 4区工程技术 Q4 CHEMISTRY, APPLIED

Chemical Industry & Chemical Engineering Quarterly Pub Date : 2021-01-01 DOI:10.2298/CICEQ201224012B

Gunay Baydar-Atak, Mert Akin Insel, M. E. Oruc, H. Sadıkoğlu

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引用次数: 0

Abstract

Platelets (PLTs) are responsible for stopping the bleeding. They are small cell fragments produced from megakaryocytes (MKs) in the bone marrow. Low platelet count is a significant health problem for a patient. PLTs can usually be stored for up to 5 days prior to transfusion. Instantaneous production of PLTs from isolated and stored MKs is crucial for the patient?s health. Thanks to microfluidic platforms, PLTs can be produced instantaneously from MKs. Herein, we have computationally studied fluid dynamics in the microchannels with slit structures and different inlet geometries. Analysis of the flow dynamics was performed by the commercial analysis software. The effects of flow rates and the angle between the inlet channels on the MKs trapping were investigated. The optimization of the angle between inlet channels and flow rates of main and pressure flows was done with Response Surface Methodology (RSM) by counting the trapped MKs. The optimum conditions lead to the percentage of trapped MKs were 100 with a relative deviation of <1%. We also concluded that flow rates to trapping a higher amount of MKs are as important as the angle between the inlet channels.

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微通道中巨核细胞捕获血小板形成的优化

血小板(PLTs)负责止血。它们是骨髓中巨核细胞(MKs)产生的小细胞碎片。血小板计数低对病人来说是一个严重的健康问题。血浆通常可在输血前储存5天。从分离和储存的mk中瞬时产生plt对患者至关重要。年代的健康。由于有了微流控平台，plt可以从mk瞬间产生。本文对狭缝结构和不同进口几何形状的微通道内的流体力学进行了计算研究。利用商业分析软件进行了流动动力学分析。研究了流量和进口通道夹角对mk捕获的影响。利用响应面法(RSM)，通过计算被困mk，对进气道夹角、主流流量和压力流流量进行优化。最佳条件下，捕获mk的百分比为100，相对偏差<1%。我们还得出结论，捕获更多mk的流量与入口通道之间的角度同样重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical Industry & Chemical Engineering Quarterly CHEMISTRY, APPLIED-ENGINEERING, CHEMICAL

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

3.3 months

期刊介绍： The Journal invites contributions to the following two main areas: • Applied Chemistry dealing with the application of basic chemical sciences to industry • Chemical Engineering dealing with the chemical and biochemical conversion of raw materials into different products as well as the design and operation of plants and equipment. The Journal welcomes contributions focused on: Chemical and Biochemical Engineering [...] Process Systems Engineering[...] Environmental Chemical and Process Engineering[...] Materials Synthesis and Processing[...] Food and Bioproducts Processing[...] Process Technology[...]