Values of alkaline phosphathase and their isoenzyme profiles in patients with cancer in respect to bone and liver metastasis

Q4 Medicine

Archive of Oncology Pub Date : 2013-01-01 DOI:10.2298/AOO1301014D

Marina Ðokic-Lisanin, V. Pantović, Z. Jovanović, G. Samardžić, V. Jurišić

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引用次数: 5

Abstract

Background: Alkaline phosphatase is a glycoprotein that catalyzes two kinds of chemical reactions: hydrolysis of phosphorus ester breaking P-O bonds and phospho-transfer reactions in which phosphoric group is transferred to an acceptor molecule. In the human body, ALP exists in multiple molecular forms whose heterogeneity is partly due to genetic factors and partly to posttranslational modifications. The aim was to evaluate a total ALP activity and its isoforms in cancer patients with bone and liver metastasis in comparison to healthy controls. Methods: Human serum was collected from 20 healthy individuals, and 20 cancer patients with bone and liver metastases, with metastases confirmed by ultrasound, computerized tomography and a radiology scan. Determination of ALP was done by the endpoint spectrophotometric method. Isoenzymes were determined by heat inactivation method. Results: In cancer patients, the total ALP activity was significantly higher (p< 0.05) compared to healthy controls. In the sera of cancer patients with liver metastases, the remaining ALP activity was two-fold higher in comparison to bone metastases. Conclusion: Determination of ALP isoenzymes is important but a correct clinical interpretation in the context of other analyses is vital for a proper diagnosis of a disease. [Projekat Ministarstva nauke Republike Srbije, br.175056]

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碱性磷酸酶及其同工酶谱在肿瘤骨和肝转移中的价值

背景:碱性磷酸酶是一种糖蛋白，它催化两种化学反应:磷酸酯水解破坏P-O键和磷酸基团转移到受体分子的磷酸转移反应。在人体内，ALP以多种分子形式存在，其异质性部分是由于遗传因素，部分是由于翻译后修饰。目的是评估骨和肝转移的癌症患者与健康对照者的总ALP活性及其亚型。方法:采集20例健康人血清和20例经超声、计算机断层扫描和放射学扫描证实为骨转移和肝转移的肿瘤患者血清。用终点分光光度法测定ALP。用热失活法测定同工酶。结果:肿瘤患者总ALP活性显著高于健康对照组(p< 0.05)。在肝转移癌患者的血清中，剩余的ALP活性是骨转移癌患者的两倍。结论:ALP同工酶的测定是重要的，但在其他分析的背景下，正确的临床解释对于疾病的正确诊断至关重要。[Srbije, br.175056]

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archive of Oncology Medicine-Oncology

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Archive of Oncology is an international oncology journal that publishes original research, editorials, review articles, case (clinical) reports, and news from oncology (medical, surgical, radiation), experimental oncology, cancer epidemiology, and prevention. Letters are also welcomed. Archive of Oncology is covered by Biomedicina Vojvodina, Biomedicina Serbica, Biomedicina Oncologica, EMBASE/Excerpta Medica, ExtraMED and SCOPUS.