Effects of mistletoe extract on markers of platelet activation and aggregation

Acta chirurgica Iugoslavica Pub Date : 2015-01-01 DOI:10.2298/ACI1502005S

T. Srdić-Rajić, A. Konić-Ristić, Nevena Tisma-Miletic, Nevena Kardum, D. Galun, J. Sikimić, M. Glibetić, M. Milićević

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Abstract

BACKGROUND: Viscum album preparations are extensively used as complementary therapy in cancer and are shown to exert antitumor activities which involve the cytotoxic properties, induction of apoptosis, inhibition of angiogenesis and several other immunomodulatory mechanisms. AIM: The aim of this study was to investigate the effects of mistletoe extract on platelet as well as monocyte functions, as an important factors in immunomodulation of cancers metastatic potencial and angiogenesis in tumors. METHODS: The effect of different concentrations of mistletoe extract on agonist-induced platelet activation markers and their aggregation with leukocytes was examined in the blood of healthy subjects (n=6) using flow cytometry. Effects on LPS -induced activation markers was determined in the blood of healthy subjects as well as on THP-1 cell line using an ELISA essays and flow cytometry. RESULTS: Mistletoe extract significantly inhibited agonist induced P selectin expression and platelet-monocytes aggregation. Additionally, mistletoe extract exerts anti-tumor effect through the stimulation of TNF-a production in LPS induced monocytes activation. CONCLUSION: Obtained data demonstrate that mistletoe extract was effective in modulating platelet and monocyte functions, as a part of pleiotropic anticancer effect. [Projekat Ministarstva nauke Republike Srbije, br. III 41030]

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槲寄生提取物对血小板活化和聚集标志物的影响

背景:粘片制剂被广泛用于癌症的辅助治疗，并被证明具有抗肿瘤活性，包括细胞毒性、诱导细胞凋亡、抑制血管生成和其他几种免疫调节机制。目的:探讨槲寄生提取物对血小板和单核细胞功能的影响，槲寄生提取物是免疫调节肿瘤转移潜能和血管生成的重要因素。方法:采用流式细胞术检测不同浓度槲寄生提取物对6例健康人血液中激动剂诱导的血小板活化标志物及其与白细胞聚集的影响。使用ELISA试剂盒和流式细胞术确定对健康受试者血液中LPS诱导的激活标记物的影响，以及对THP-1细胞系的影响。结果:槲寄生提取物显著抑制激动剂诱导的P选择素表达和血小板单核细胞聚集。此外，槲寄生提取物通过刺激LPS诱导的单核细胞活化中TNF-a的产生而发挥抗肿瘤作用。结论:槲寄生提取物具有调节血小板和单核细胞功能的作用，是其多效抗癌作用的一部分。[南斯拉夫]塞族共和国部长项目;三世41030]

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Acta chirurgica Iugoslavica

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