Anticancer and apoptotic effects of a polysaccharide extract isolated from Lactarius chrysorrheus Fr. in HepG2 and PANC-1 cell lines

IF 0.7 4区生物学 Q4 BIOLOGY

Archives of Biological Sciences Pub Date : 2022-01-01 DOI:10.2298/abs220803030m

Dogukan Mutlu, C. Cakir, M. Ozturk, Ş. Arslan

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引用次数: 2

Abstract

Mushrooms are widely used in many cultures for nutritional and health benefits. The Lactarius species is found in the Aegean region of Turkey. Lactarius chrysorrheus Fr. is a wild mushroom that contains a milky juice. In this study, we investigated the in vitro cytotoxic potential and apoptotic effect of the polysaccharide extract prepared from L. chrysorrheus by water extraction and alcohol precipitation using the tetrazolium MTT dye assay, annexin V staining, wound healing and colony formation, and qRT-PCR. The molecular weights of three peaks observed in HPLC chromatograms were calculated as 1869.9, 3043.92 and 16821.47 Da. The extract exhibited cytotoxic activity at 72 h, with an IC50 value of 296.42 ?g/mL in HepG2 and 444.43 ?g/mL in PANC-1 cells; the extract that was tested on the normal HEK293 cell line exhibited no cytotoxicity. Further, L. chrysorrheus upregulated the expression of CASPASE 3 and CASPASE 9 while downregulating B-cell lymphoma 2 (BCL-2) and B-cell lymphoma-extra large (Bcl-xL) genes, and inhibited cell migration and colony formation in HepG2 and PANC-1 cells. This study provides new insight into the use of the polysaccharide from L. chrysorrheus in the development of novel anticancer agents.

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乳酸菌多糖提取物对HepG2和PANC-1细胞株的抗癌和凋亡作用

蘑菇在许多文化中被广泛用于营养和健康。该物种是在土耳其的爱琴海地区发现的。乳酸菌是一种含有乳白色汁液的野生蘑菇。本研究采用四氮唑MTT染色、膜联蛋白V染色、伤口愈合和菌落形成、qRT-PCR等方法，研究了水提醇沉法制备的金钱菊多糖提取物的体外细胞毒潜能和细胞凋亡作用。HPLC图中3个峰的分子量分别为1869.9、3043.92和16821.47 Da。72 h时显示细胞毒活性，对HepG2和PANC-1细胞的IC50值分别为296.42 μ g/mL和444.43 μ g/mL;提取液对正常HEK293细胞系无细胞毒性作用。此外，白羊草上调CASPASE 3和CASPASE 9的表达，下调b细胞淋巴瘤2 (BCL-2)和b细胞淋巴瘤-特大(Bcl-xL)基因的表达，抑制HepG2和PANC-1细胞的细胞迁移和集落形成。本研究为利用金菊多糖开发新型抗癌药物提供了新的思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Biological Sciences 生物-生物学

CiteScore

1.40

自引率

0.00%

发文量

审稿时长

3-8 weeks

期刊介绍： The Archives of Biological Sciences is a multidisciplinary journal that covers original research in a wide range of subjects in life science, including biology, ecology, human biology and biomedical research. The Archives of Biological Sciences features articles in genetics, botany and zoology (including higher and lower terrestrial and aquatic plants and animals, prokaryote biology, algology, mycology, entomology, etc.); biological systematics; evolution; biochemistry, molecular and cell biology, including all aspects of normal cell functioning, from embryonic to differentiated tissues and in different pathological states; physiology, including chronobiology, thermal biology, cryobiology; radiobiology; neurobiology; immunology, including human immunology; human biology, including the biological basis of specific human pathologies and disease management.