Cerium oxide nanoparticles mitigate retinal pigment epithelium (RPE) death using APRE19 cell model

IF 1.4 Q4 NANOSCIENCE & NANOTECHNOLOGY
E. Dahl, N. Efstathiou, Amit K. Roy
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引用次数: 0

Abstract

Objective(s): In this study, we present the potential of cerium oxide nanoparticle pretreatment on ARPE-19 cells, a cell line of the Retinal Pigment Epithelium (RPE), as a therapeutic modality to cellular stresses such as low serum starvation.Materials and Methods: ARPE-19 cells were pretreated with nano-cerium oxide at a concentration of 500 µg/mL before low serum stress was induced for 24, 48, 72, and 96 hours. Starvation stress was induced by using low concentrations of Fetal Bovine Serum (FBS) media at three increments: 10%, 1%, 0.1%.Results: Contrast images demonstrated higher cell confluence and cell integrity in cells pretreated with cerium oxide nanoparticles compared to untreated cells. Increased cell viability for cerium oxide pretreated cells was confirmed by MTS assay after 96 hours of serum starvation.Conclusion: By using nanoparticles to influence pathways of apoptosis, we hope to rescue ARPE-19 cells from a range of stressors, including oxidative stress, and re-establish homeostasis for the cell. Nanoparticles may represent a novel class of therapeutics for diseases of the eye, like AMD and blue-light induced oxidative stress.
氧化铈纳米颗粒减轻APRE19细胞模型视网膜色素上皮(RPE)死亡
目的:在本研究中,我们展示了氧化铈纳米颗粒预处理视网膜色素上皮(RPE)细胞系ARPE-19细胞的潜力,作为细胞应激(如低血清饥饿)的治疗方式。材料与方法:用500µg/mL浓度的纳米氧化铈预处理ARPE-19细胞,诱导低血清应激24、48、72、96小时。采用低浓度胎牛血清(FBS)培养基,分别以10%、1%、0.1%的添加量诱导饥饿应激。结果:对比图像显示,与未处理的细胞相比,氧化铈纳米颗粒预处理的细胞具有更高的细胞融合和细胞完整性。血清饥饿96小时后,MTS实验证实氧化铈预处理细胞的细胞活力增加。结论:我们希望通过纳米颗粒影响细胞凋亡途径,将ARPE-19细胞从包括氧化应激在内的一系列应激源中拯救出来,并重建细胞的内稳态。纳米颗粒可能代表了一种新的眼部疾病治疗方法,如AMD和蓝光诱导的氧化应激。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Nanomedicine Journal
Nanomedicine Journal NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
3.40
自引率
0.00%
发文量
0
审稿时长
12 weeks
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