Chronic resveratrol reverses a mild angiotensin II-induced pressor effect in a rat model.

IF 1.5 Q3 PERIPHERAL VASCULAR DISEASE
Integrated Blood Pressure Control Pub Date : 2016-01-28 eCollection Date: 2016-01-01 DOI:10.2147/IBPC.S96092
Kevin L Gordish, William H Beierwaltes
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引用次数: 8

Abstract

Resveratrol is reported to reduce blood pressure in animal models of hypertension, but the mechanisms are unknown. We have shown that resveratrol infusion increases sodium excretion. We hypothesized that chronic ingestion of resveratrol would reduce angiotensin II (Ang II)-induced increases in blood pressure by decreasing oxidative stress and by also decreasing sodium reabsorption through a nitric oxide-dependent mechanism. We infused rats with vehicle or 80 μg Ang II/d over 4 weeks. Vehicle or Ang II-infused rats were individually housed, pair fed, and placed on a diet of normal chow or normal chow plus 146 mg resveratrol/d. Groups included 1) control, 2) resveratrol-fed, 3) Ang II-treated, and 4) Ang II plus resveratrol. Systolic blood pressure was measured by tail cuff. During the 4th week, rats were placed in metabolic caging for urine collection. NO2/NO3 and 8-isoprostane excretion were measured. Ang II increased systolic blood pressure in the 1st week by +14±5 mmHg (P<0.05) in Group 3 and +10±3 mmHg (P<0.05) in Group 4, respectively. Blood pressure was unchanged in Groups 1 and 2. After 4 weeks, blood pressure remained elevated in Group 3 rats with Ang II (+9±3 mmHg, P<0.05), but in Group 4, blood pressure was no longer elevated (+2±2 mmHg). We found no significant differences between the groups in sodium excretion or cumulative sodium balance (18.49±0.12, 17.75±0.16, 17.97±0.17, 18.46±0.18 μEq Na+/7 d in Groups 1-4, respectively). Urinary excretion of NO2/NO3 in the four groups was 1) 1631±207 μmol/24 h, 2) 1045±236 μmol/24 h, 3) 1490±161 μmol/24 h, and 4) 609±17 μmol/24 h. 8-Isoprostane excretion was 1) 63.85±19.39 nmol/24 h, 2) 73.57±22.02 nmol/24 h, 3) 100.69±37.62 nmol/24 h, and 4) 103.00±38.88 nmol/24 h. We conclude that chronic resveratrol supplementation does not blunt Ang II-increased blood pressure, and while resveratrol has mild depressor effects, these do not seem to be due to natriuresis or enhanced renal nitric oxide synthesis.

慢性白藜芦醇在大鼠模型中逆转轻度血管紧张素ii诱导的降压作用。
据报道,白藜芦醇可以降低高血压动物模型的血压,但其机制尚不清楚。我们已经证明白藜芦醇输注会增加钠的排泄。我们假设慢性摄入白藜芦醇可以通过减少氧化应激和通过一氧化氮依赖机制减少钠重吸收来降低血管紧张素II (Ang II)诱导的血压升高。给大鼠灌胃80 μg Ang II/d,持续4周。对照大鼠或注射了angii的大鼠单独饲养,成对饲养,分别饲喂正常饲料或正常饲料加146 mg白藜芦醇/d的饮食。各组包括:1)对照组,2)白藜芦醇组,3)angii组,4)angii加白藜芦醇组。用尾袖测量收缩压。第4周,将大鼠置于代谢笼中收集尿液。测定NO2/NO3和8-异前列腺素排泄量。Angⅱ使第1周收缩压升高+14±5 mmHg (P
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来源期刊
Integrated Blood Pressure Control
Integrated Blood Pressure Control PERIPHERAL VASCULAR DISEASE-
CiteScore
4.60
自引率
0.00%
发文量
13
审稿时长
16 weeks
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