Isolation and Identification of Antiradical and Anti-photooxidant Component of Aloe vera chinensis

Y. Dewi, T. Tranggono, S. Raharjo, Puji Hastuti
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引用次数: 0

Abstract

A potent antiradical and antiphotooxidant compound from Aloe vera chinensis was investigated. The results indicated that the methanolic extracts produced stronger antiradical and antiphotoaxidant activity also gave higher yields of extract than other organic solvents. Silica gel column separated methanolic extracts into five fractions. Of the five fractions, fraction II possessed significant antiradical activity (70.2 % inhibition on free radical DPPH) and its showed significant antiphotooxidant activity (60.1 % inhibition on hydroperoxide formation). Subsequently, fraction II was separated into eight subfractions by silica gel column. The sub fraction II-b exhibited stronger antiradical and antiphotooxidant activity than others and showed a single spot in thin layer chromatography Silica gel 60 F 254 plates. On the basis of mass spectrophotometry in combination with reversed phased, high performance chromatography, this compound has been identified as 1,8 dihydroxy-3- hydroxymethylanthraquinone (Aloe emodin).
芦荟抗自由基和抗光氧化成分的分离与鉴定
从芦荟中提取了一种有效的抗自由基和抗光氧化化合物。结果表明,甲醇提取物具有较强的抗自由基和抗光氧化活性,且提取率高于其他有机溶剂。硅胶柱将甲醇提取物分离成五个部分。其中,ⅱ组分对自由基DPPH的抑制率为70.2%,对过氧化氢的抑制率为60.1%。随后,用硅胶柱将组分II分离成8个亚组分。亚组分II-b具有较强的抗自由基和抗光氧化活性,在薄层色谱硅胶60f254板上呈现单斑点。通过质谱联用反相高效色谱法鉴定该化合物为1,8二羟基-3-羟甲基蒽醌(芦荟大黄素)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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