Micropropagation of Achillea millefolium L. on half-strength MS medium and direct rooting and acclimatization of microshoots in hydroponic culture.

Glasnik Sumarskog Fakulteta Univerzitet u Beogradu Pub Date : 2015-01-01 DOI:10.2298/gsf1511099m

M. Marković, D. Skočajić, M. Grbic, M. Djukic, D. Obratov-Petković, Danijela Djunisijevic-Bojovic, Milica Borovica

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引用次数: 1

Abstract

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution. [Projekat Ministarstva nauke Republike Srbije, br. 43007]

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半强MS培养基上千叶水蛭的微繁殖及微芽水培直接生根驯化。

本研究的目的是确定药用植物千叶在半强MS培养基上进行微繁殖的可能性，并对获得的微芽进行离体生根和水培驯化，以建立一种高效的生产方法。外植体分为基插和顶插两种，以顶插效果较好。繁殖期新梢发育成功，再生率达100%。在改良的Hoagland营养液中离体生根成功率为83%，但在无激素的半强度MS培养基上离体生根率更高(95%)。但是，考虑到当排除离体生根阶段时，千叶草的大规模生产效率更高，因此可以推荐这种方法用于该药用植物的商业繁殖。为了优化水培营养液的组成、EC和pH值，有必要进行进一步的研究。[南斯拉夫]塞族共和国部长项目;43007]

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Glasnik Sumarskog Fakulteta Univerzitet u Beogradu

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17 weeks