Detection of carbapenem-resistance and biofilm formation genes, and genetic relatedness of Acinetobacter baumannii isolates

4区农林科学 Q3 Agricultural and Biological Sciences

Genetika-Belgrade Pub Date : 2022-01-01 DOI:10.2298/gensr2203069s

M. Simonović, Z. Lepšanović, B. Rakonjac, Srdjan Lazić

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引用次数: 0

Abstract

Acinetobacter baumannii is one of the most important nosocomial pathogen worldwide. This study aimed to investigate the virulence potential and genomic relatedness of A. baumannii strains isolated from patients hospitalized in the Military Medical Academy (MMA) by detecting OXA-type carbapenemases genes, biofilm-associated genes, and by RAPD analysis. PCR was used to detect the blaoxa genes, ISAba-1 genetic element, and biofilm-associated genes. The genomic relatedness was determined by RAPD analysis using four different primers (AP2, DAF4. M13, and DECA). blaoxa-51-like, blaoxa-23-like, blaoxa-24-like, and blaoxa-58-like were present in 100%, 34.0%, 62.4%, and 3.1% of isolates, respectively. All isolates had the ISAba1 sequence in their genome, in 35.1% of isolates it was associated with the blaoxa-51-like, and in 97.0% with the blaoxa-23-like gene. Biofilm-associated genes bap, ompA, epsA, csuA/BABCDE, and pgaABCD were detected in 93.8%, 95.8%, 88.1%, 98.4%, and 98.9% isolates, respectively. RAPD analysis showed a high degree of genome similarity and clonal dispersion of the isolates. Detection of blaoxa genes, especially biofilm-associated genes, in a high percentage of A. baumannii isolates indicated their great pathogenic potential. RAPD analysis revealed a high level of genomic similarity and clonal dispersion of the majority of isolates through MMA. Further, a continuous introduction of individual strains with different profiles contributes to the genetic diversity of A. baumannii isolates. These results can be useful for further management and tracking nosocomial outbreaks.

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鲍曼不动杆菌碳青霉烯耐药和生物膜形成基因检测及遗传亲缘关系

鲍曼不动杆菌是世界上最重要的医院致病菌之一。本研究旨在通过检测oxa型碳青霉烯酶基因、生物膜相关基因和RAPD分析，探讨从军事医学学院(MMA)住院患者中分离的鲍曼不动杆菌的毒力潜力和基因组相关性。PCR检测blaxa基因、ISAba-1遗传元件和生物膜相关基因。使用4种不同的引物(AP2、DAF4和DAF4)进行RAPD分析，确定基因组亲缘性。M13和DECA)。blaoxa-51 like、blaoxa-23 like、blaoxa-24 like和blaoxa-58 like分别为100%、34.0%、62.4%和3.1%。所有分离株基因组中均含有ISAba1序列，其中35.1%的分离株与blaoxa-51样基因相关，97.0%的分离株与blaoxa-23样基因相关。生物膜相关基因bap、ompA、epsA、csuA/BABCDE和pgaABCD的检出率分别为93.8%、95.8%、88.1%、98.4%和98.9%。RAPD分析表明，分离株具有高度的基因组相似性和克隆分散性。鲍曼不动杆菌分离株中检测到的bloxa基因，特别是与生物膜相关的基因，表明其具有很大的致病潜力。RAPD分析显示，大多数分离株通过MMA具有较高的基因组相似性和克隆分散性。此外，不断引入具有不同特征的单个菌株有助于鲍曼不动杆菌分离株的遗传多样性。这些结果可用于进一步管理和跟踪医院暴发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetika-Belgrade AGRONOMY-GENETICS & HEREDITY

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The GENETIKA is dedicated to genetic studies of all organisms including genetics of microorganisms, plant genetics, animal genetics, human genetics, molecular genetics, genomics, functional genomics, plant and animal breeding, population and evolutionary genetics, mutagenesis and genotoxicology and biotechnology.