Detection and quantification of pathogenic microorganisms in patients with chronic periodontitis before and after treatment with chlorhexidine digluconate

4区农林科学 Q3 Agricultural and Biological Sciences

Genetika-Belgrade Pub Date : 2022-01-01 DOI:10.2298/gensr2201491j

Katarina Jovanovic, J. Carkic, N. Nikolic, Nada Novakovic, M. Hadzi-Mihailović, E. Čolak, S. Čakić, J. Milašin

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Abstract

The aim of this study was to evaluate the efficiency of chlorhexidine digluconate solution (CHX) as an adjunct to nonsurgical therapy (NPT) of patients with chronic periodontitis (CP), by analyzing the presence and quantity of periopathogenic microorganisms in subgingival biofilm. DNA was extracted from the subgingival biofilm obtained from 40 patients with CP divided into two groups (NPT+CHX and NPT alone as control) at baseline and 2 months after the therapy. The presence of selected periodontal pathogens (A. actinomycetemcomitans, P. gingivalis, E. corrodens, T. denticola, and T. forsythia) was determined by polymerase chain reaction (PCR), while total bacterial load was assessed by quantitative PCR. The incidence of microorganisms decreased following treatment, both with NPT+CHX and NPT alone, but without reaching statistically significant difference in the NPT group. In the NPT+CHX group, a significant reduction of prevalence of two species: T. denticola (P = 0.008) and T. forsythia (P = 0.016), as well as of total microorganism count (P = 0.002) was observed two months after treatment. In conclusion, the present findings support the use of CHX as adjunctive therapy in CP.

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双光酸氯己定治疗前后慢性牙周炎患者病原微生物的检测与定量

本研究的目的是通过分析牙龈下生物膜中周围病原菌的存在和数量，评估双光酸氯己定溶液(CHX)作为非手术治疗慢性牙周炎(CP)患者的辅助治疗(NPT)的有效性。在基线和治疗后2个月，将40例CP患者分为两组(NPT+CHX和NPT单独为对照组)，从龈下生物膜中提取DNA。采用聚合酶链反应(PCR)法检测牙周病原菌(放线菌、牙龈卟啉卟啉、锈蚀菌、齿牙卟啉卟啉和连翘卟啉卟啉)的存在情况，并采用定量PCR法评估细菌总负荷。无论NPT+CHX还是NPT单独治疗，治疗后微生物的发生率均有所下降，但NPT组间差异无统计学意义。在NPT+CHX组中，治疗2个月后，两种细菌的患病率显著降低:牙齿T. (P = 0.008)和连翘T. (P = 0.016)，微生物总数显著减少(P = 0.002)。总之，目前的研究结果支持使用CHX作为CP的辅助治疗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetika-Belgrade AGRONOMY-GENETICS & HEREDITY

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The GENETIKA is dedicated to genetic studies of all organisms including genetics of microorganisms, plant genetics, animal genetics, human genetics, molecular genetics, genomics, functional genomics, plant and animal breeding, population and evolutionary genetics, mutagenesis and genotoxicology and biotechnology.