Genetic diversity and relationships among salvia species by ISSR markers

4区农林科学 Q3 Agricultural and Biological Sciences

Genetika-Belgrade Pub Date : 2021-01-01 DOI:10.2298/gensr2102559y

Zhao Yanpeng, W. Hongmei, Liang Wei, M. Khayatnezhad, F. Faisal

引用次数: 1

Abstract

Species identification is fundamentally important within the fields of conservation, biology, biogeography and ecology. Salvia species are herbaceous, biennial or annual, strongly aromatic. Inter-Simple sequence repeats (ISSR) molecular markers were used for evaluate genetic diversity and relationship analysis of 30 Salvia species. Ten selected ISSR primers amplified 116 loci, respectively, of which all were polymorphic. The obtained average polymorphism information content 0.39, average band informativeness 10.5 and the marker index 3.1 revealed high genetic diversity prevailing among Salvia accessions. The dendrogram was constructed based on ISSR separated the individuals into sub-clusters in accordance with their species. Our results indicated that ISSR markers can be used as a reliable and informative technique for evaluation of genetic diversity and relationships among Salvia species. The objectives of present study are: 1) can ISSR markers identify Salvia species, 2) what is the genetic of these taxa in Iran, and 3) to investigate the species inter-relationship?

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鼠尾草种间遗传多样性及亲缘关系

物种鉴定在保护、生物学、生物地理学和生态学等领域具有重要意义。鼠尾草属草本，二年生或一年生，芳香强烈。采用ISSR (Inter-Simple sequence repeats)分子标记对30个鼠尾草品种进行了遗传多样性评价和亲缘关系分析。10条ISSR引物分别扩增出116个位点，均为多态性位点。获得的平均多态性信息含量为0.39，平均频带信息含量为10.5，标记指数为3.1，表明各鼠尾草材料具有较高的遗传多样性。在ISSR的基础上构建了树形图，将不同种类的个体划分为不同的子聚类。结果表明，ISSR标记可作为评价鼠尾草品种间遗传多样性和亲缘关系的一种可靠的信息技术。本研究的目的是:1)ISSR标记能否识别鼠尾草属;2)鼠尾草属在伊朗的遗传机制;3)探讨鼠尾草属间的相互关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetika-Belgrade AGRONOMY-GENETICS & HEREDITY

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The GENETIKA is dedicated to genetic studies of all organisms including genetics of microorganisms, plant genetics, animal genetics, human genetics, molecular genetics, genomics, functional genomics, plant and animal breeding, population and evolutionary genetics, mutagenesis and genotoxicology and biotechnology.