Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models

IF 0.2 4区农林科学 Q2 Veterinary

Veterinaria Mexico Pub Date : 2016-12-09 DOI:10.21753/VMOA.3.4.366

Cristina Ibarra Zazueta, B. A. Reynoso, R. Castro, E. G. P. Resendiz, E. D. Aparicio

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引用次数: 1

Abstract

Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Ibarra Zazueta C, Arellano Reynoso B, Hernandez Castro R, Palomares Resendiz EG, Diaz Aparicio E. Evaluation of the aroA mutant of Corynebacterium pseudotuberculosis in cellular and murine models. Veterinaria Mexico OA. 2016;3(4). doi: 10.21753/vmoa.3.4.366 Caseous lymphadenitis of small ruminants causes economic losses worldwide. To date, no effective vaccine has been developed against the causative agent of this disease, Corynebacterium pseudotuberculosis . The objective of the present work was to evaluate an aroA mutant gene strain of C. pseudotuberculosis in cellular and murine models, for attenuation and the ability to stimulate an immune response. The intracellular survival of the aroA mutant strain and the wild type strain (WT) of C. pseudotuberculosis was evaluated in J774A.1 murine macrophages using a multiplicity of infection (MOI) of 1:1 with the following infection times: 30 min, and 1, 2, 4, 8, 12, and 24 h. The largest difference in the intracellular survival of the mutant was observed 30 min post-infection. After subcutaneous skin vaccination, the subcutaneous lesion progression observed on the 14 th day was more severe in those animals that were vaccinated with the WT strain. An analysis of the residual virulence in the murine model did not reveal any bacteria in mice vaccinated with the aroA strain on day 28 post-vaccination. Mice vaccinated with the mutant showed 50 % protection against the intraperitoneal challenge, exceeding that of the control group (41.67 %). We conclude that the virulence of the aroA mutant was significantly attenuated in both cellular and murine models according to the residual virulence detected in mice. However, vaccination with the mutant failed to confer at least 80 % protection, which is desirable for an immunogen. Hence, this study contributes to the knowledge of the immune response against Corynebacterium pseudotuberculosis .

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假结核棒状杆菌aroA突变体在细胞和小鼠模型中的评价

引用本文:Ibarra Zazueta C, Arellano Reynoso B, Hernandez Castro R, Palomares Resendiz EG, Diaz Aparicio E.假结核棒状杆菌aroA突变体在细胞和小鼠模型中的评价。墨西哥兽医局。2016; 3(4)。小反刍动物干酪样淋巴结炎在世界范围内造成经济损失。迄今为止，还没有开发出有效的疫苗来对抗这种疾病的病原体——假结核棒状杆菌。本研究的目的是在细胞和小鼠模型中评估假结核杆菌aroA突变基因株的衰减和刺激免疫反应的能力。在J774A中对aroA突变菌株和野生型菌株(WT)的细胞内存活进行了评价。1小鼠巨噬细胞使用1:1的感染倍数(MOI)，感染时间为:30min, 1,2,4,8,12和24h。在感染后30min观察到突变体的细胞内存活差异最大。皮下接种后，第14天观察到接种WT菌株的动物皮下病变进展更为严重。对小鼠模型的残留毒力分析显示，接种aroA菌株的小鼠在接种后28天未发现任何细菌。接种突变体的小鼠对腹腔内攻击的保护率为50%，超过对照组(41.67%)。根据在小鼠中检测到的残余毒力，我们得出结论，aroA突变体在细胞和小鼠模型中的毒力都显著减弱。然而，用突变体接种疫苗不能提供至少80%的保护，这是免疫原所期望的。因此，本研究有助于了解对假结核棒状杆菌的免疫反应。

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来源期刊

Veterinaria Mexico VETERINARY SCIENCES-

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).