A single neonatal administration of soybean oil and/or tamoxifen affects permanently the testis histomorphology in adult rats.

IF 0.2 4区 农林科学 Q2 Veterinary
Alicia González-González, E. González-Padilla, F. Fierro-Fierro, M. D. L. Juárez-Mosqueda, J. Pérez-Rivero, M. Vergara-Onofre
{"title":"A single neonatal administration of soybean oil and/or tamoxifen affects permanently the testis histomorphology in adult rats.","authors":"Alicia González-González, E. González-Padilla, F. Fierro-Fierro, M. D. L. Juárez-Mosqueda, J. Pérez-Rivero, M. Vergara-Onofre","doi":"10.21753/VMOA.3.2.364","DOIUrl":null,"url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Gonzalez Gonzalez A, Gonzalez Padilla E, Fierro Fierro F, Juarez Mosqueda M de L, Perez Rivero JJ, Vergara Onofre M. A single neonatal administration of soybean oil and/or tamoxifen affects permanently the testis histomorphology in adult rats. Veterinaria Mexico OA. 2016;3(2). doi: 10.21753/vmoa.3.2.364 The aim of this study was to determine the effect of tamoxifen (Tx) and its vehicle, soybean oil (SO), during the critical period of hypothalamic sexual differentiation in newborn male rats, regarding gonadal histomorphology during adulthood. The animals were randomly divided into 3 groups (n = 5 each). An hour after birth, one group was treated subcutaneously with 200 μg of Tx, using commercial SO (20 uL) as a vehicle; another group was treated with only 20 μL of SO; the control group received no treatment. All rats were weighed and sacrificed by cervical dislocation on day 90 post-treatment. Testicles were removed, weighed and processed for histological evaluation. The single administration of Tx and/or SO during the critical period of sexual differentiation of the hypothalamus permanently altered testicular histomorphology, spermatogenesis, and body weight in adulthood. Alterations included vacuolization and reduction in the number of spermatogonia and Sertoli cells. The administration of Tx reduced the testicular weight, the diameter and area of the seminiferous tubules, and the height of the germinal epithelium, and increased the intertubular space. Soybean oil by itself reduced the number of spermatocytes and spermatids more than Tx did. There was no effect on the number of Leydig cells. The possibility that soybean oil can act as an endocrine disruptor deserves greater attention and opens the possibility for the development of new methods of pest control. Figure 1. Representative hematoxylin-eosin-stained histological sections of testicles collected from the control group (A y B), rats treated with SO (C y D) and rats treated with Tx (E y F). A, C and E, 200X. B, D and F, 400X. T: Tubule. LC: Leydig cells. Histological changes induced by Tx and/or SO treatments can be observed. Black arrows point to the largest intertubular space. White arrows in E and F indicate the ungrouping of the LC. Asterisks indicate the vacuoles in the seminiferous tubules.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2000,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.3.2.364","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinaria Mexico","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.21753/VMOA.3.2.364","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Veterinary","Score":null,"Total":0}
引用次数: 0

Abstract

Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Gonzalez Gonzalez A, Gonzalez Padilla E, Fierro Fierro F, Juarez Mosqueda M de L, Perez Rivero JJ, Vergara Onofre M. A single neonatal administration of soybean oil and/or tamoxifen affects permanently the testis histomorphology in adult rats. Veterinaria Mexico OA. 2016;3(2). doi: 10.21753/vmoa.3.2.364 The aim of this study was to determine the effect of tamoxifen (Tx) and its vehicle, soybean oil (SO), during the critical period of hypothalamic sexual differentiation in newborn male rats, regarding gonadal histomorphology during adulthood. The animals were randomly divided into 3 groups (n = 5 each). An hour after birth, one group was treated subcutaneously with 200 μg of Tx, using commercial SO (20 uL) as a vehicle; another group was treated with only 20 μL of SO; the control group received no treatment. All rats were weighed and sacrificed by cervical dislocation on day 90 post-treatment. Testicles were removed, weighed and processed for histological evaluation. The single administration of Tx and/or SO during the critical period of sexual differentiation of the hypothalamus permanently altered testicular histomorphology, spermatogenesis, and body weight in adulthood. Alterations included vacuolization and reduction in the number of spermatogonia and Sertoli cells. The administration of Tx reduced the testicular weight, the diameter and area of the seminiferous tubules, and the height of the germinal epithelium, and increased the intertubular space. Soybean oil by itself reduced the number of spermatocytes and spermatids more than Tx did. There was no effect on the number of Leydig cells. The possibility that soybean oil can act as an endocrine disruptor deserves greater attention and opens the possibility for the development of new methods of pest control. Figure 1. Representative hematoxylin-eosin-stained histological sections of testicles collected from the control group (A y B), rats treated with SO (C y D) and rats treated with Tx (E y F). A, C and E, 200X. B, D and F, 400X. T: Tubule. LC: Leydig cells. Histological changes induced by Tx and/or SO treatments can be observed. Black arrows point to the largest intertubular space. White arrows in E and F indicate the ungrouping of the LC. Asterisks indicate the vacuoles in the seminiferous tubules.
单次给予大豆油和/或他莫昔芬对成年大鼠睾丸组织形态学有永久性影响。
引用本文:Gonzalez Gonzalez A, Gonzalez Padilla E, Fierro Fierro F, Juarez Mosqueda M de L, Perez Rivero JJ, Vergara Onofre M.单次给予大豆油和/或他莫西芬对成年大鼠睾丸组织形态学的影响。墨西哥兽医局。2016; 3(2)。本研究的目的是确定他莫昔芬(Tx)及其代药大豆油(SO)在新生雄性大鼠下丘脑性别分化关键时期对成年期性腺组织形态学的影响。随机分为3组,每组5只。出生1 h后,一组以商业SO (20 uL)为载体,皮下注射Tx 200 μg;另一组仅用20 μL SO处理;对照组不进行任何治疗。治疗后第90天称重,颈椎脱臼处死。取下睾丸,称重并进行组织学评估。在下丘脑性别分化的关键时期单次给予Tx和/或SO会永久性地改变成年期睾丸组织形态、精子发生和体重。改变包括空泡化和精原细胞和支持细胞数量的减少。Tx可降低睾丸重量、精小管直径和面积、生殖上皮高度,增大精小管间距。大豆油本身比Tx更能减少精母细胞和精母细胞的数量。对间质细胞的数量没有影响。大豆油作为内分泌干扰物的可能性值得更多的关注,并为开发新的害虫防治方法提供了可能性。图1所示。对照组(A y B)、SO组(C y D)和Tx组(E y F)的睾丸组织切片,有代表性的苏木精-伊红染色。B, D, F, 400X。师:小管。间质细胞。可以观察到Tx和/或SO处理引起的组织学改变。黑色箭头指向最大的管间空间。E和F中的白色箭头表示LC未分组。星号表示精管中的液泡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Veterinaria Mexico
Veterinaria Mexico VETERINARY SCIENCES-
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信