Genome Organization of the Three Identical ATP1 Genes on the Left Arm of Chromosome II of Saccharomyces cerevisiae: Sequence Analysis of the 35-kb Region Containing Three ATP1 Genes

Abstract

The ATP1 gene (YBL099w) encoding the F1F0-ATPase complex  subunit of Saccharomyces cerevisiae is present on the left arm close to the telomere of chromosome II, and only one copy was reported by the Genome Project. Recently, we reported that three ATP1s designated ATP1a, ATP1b and ATP1c are located on chromosome II, with different distances between ATP1a and ATP1b, and ATP1b and ATP1c as identified previously. To elucidate the ATP1 repetition and their junction sites, we report here the complete nucleotide sequence of this region (approximately 35 kb) on the left arm of chromosome II in Saccharomyces cerevisiae using the prime clones 70113 and 70804 from ATCC, and genomic DNAs from various yeast strains S288C, DC5, W303-1A and those of the gene copy-specific ATP1-disruptants. The nucleotide sequences of the three ATP1s were identical, and they were repeated along with differing amounts of neighboring DNA sequences. The nucleotide sequence of the ATP1 repeat region has been deposited to DDBJ (AB304259, 20070511181828.08607). Keyword: ATP1, Nucleotide sequence, Repetitive genes, Junction, Saccharomyces cerevisiae.