Manganese-enhanced MRI Offers Correlation with Severity of Spinal Cord Injury in Experimental Models

Q4 Medicine
N. Martirosyan, G. Turner, J. Kaufman, Arpan A. Patel, E. Belykh, M. Kalani, N. Theodore, M. Preul
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引用次数: 5

Abstract

Background: Spinal cord injuries (SCI) are clinically challenging, because neural regeneration after cord damage is unknown. In SCI animal models, regeneration is evaluated histologically, requiring animal sacrifice. Noninvasive techniques are needed to detect longitudinal SCI changes. Objective: To compare manganese-enhanced magnetic resonance imaging (MRI [MEMRI]) in hemisection and transection of SCI rat models with diffusion tensor imaging (DTI) and histology. Methods: Rats underwent T9 spinal cord transection (n=6), hemisection (n=6), or laminectomy without SCI (controls, n=6). One-half of each group received lateral ventricle MnCl2 injections 24 hours later. Conventional DTI or T1-weighted MRI was performed 84 hours post-surgery. MEMRI signal intensity ratio above and below the SCI level was calculated. Fractional anisotropy (FA) measurements were taken 1 cm rostral to the SCI. The percentage of FA change was calculated 10 mm rostral to the SCI epicenter, between FA at the dorsal column lesion normalized to a lateral area without FA change. Myelin load (percentage difference) among groups was analyzed by histology. Results: In transection and hemisection groups, mean MEMRI ratios were 0.62 and 0.87, respectively, versus 0.99 in controls (P<0.001 and P<0.001, respectively); mean FA decreases were 67.5% and 40.1%, respectively, compared with a 6.1% increase in controls (P=0.002 and P=0.019, respectively). Mean myelin load decreased by 38.8% (transection) and 51.8% (hemisection) compared to controls (99.1%) (P<0.001 and P<0.001, respectively). Pearson’s correlation coefficients were -0.94 for MEMRI ratio and FA changes and 0.87 for MEMRI and myelin load. Conclusion: MEMERI results correlated to SCI severity measured by FA and myelin load. MEMRI is a useful noninvasive tool to assess neuronal damage after SCI.
实验模型中锰增强MRI与脊髓损伤严重程度的相关性
背景:脊髓损伤(SCI)在临床上具有挑战性,因为脊髓损伤后神经再生是未知的。在脊髓损伤动物模型中,通过组织学评估再生,需要动物牺牲。需要无创技术来检测脊髓纵向变化。目的:比较脊髓损伤大鼠半切和横断的锰增强磁共振成像(MRI [MEMRI])与弥散张量成像(DTI)和组织学。方法:大鼠分别行T9脊髓横断(n=6)、半断(n=6)或无脊髓损伤的椎板切除术(n=6)。24小时后,两组各有一半小鼠接受侧脑室MnCl2注射。术后84小时行常规DTI或t1加权MRI检查。计算SCI水平上下MEMRI信号强度比。分数各向异性(FA)测量在脊髓侧侧1cm处进行。计算脊髓损伤震中吻侧10毫米处FA变化的百分比,将背柱病变的FA归一化到没有FA变化的外侧区域。组织学分析各组髓磷脂负荷(百分比差异)。结果:横切组和半切组的平均MEMRI比值分别为0.62和0.87,对照组为0.99 (P<0.001和P<0.001);平均FA分别减少67.5%和40.1%,而对照组增加6.1% (P=0.002和P=0.019)。与对照组(99.1%)相比,平均髓磷脂负荷下降了38.8%(横切)和51.8%(半切)(P<0.001和P<0.001)。MEMRI比率与FA变化的Pearson相关系数为-0.94,MEMRI与髓磷脂负荷的Pearson相关系数为0.87。结论:MEMERI结果与FA和髓磷脂负荷测量的SCI严重程度相关。MEMRI是评估脊髓损伤后神经元损伤的一种有用的无创工具。
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来源期刊
Open Neuroimaging Journal
Open Neuroimaging Journal Medicine-Radiology, Nuclear Medicine and Imaging
CiteScore
0.70
自引率
0.00%
发文量
3
期刊介绍: The Open Neuroimaging Journal is an Open Access online journal, which publishes research articles, reviews/mini-reviews, and letters in all important areas of brain function, structure and organization including neuroimaging, neuroradiology, analysis methods, functional MRI acquisition and physics, brain mapping, macroscopic level of brain organization, computational modeling and analysis, structure-function and brain-behavior relationships, anatomy and physiology, psychiatric diseases and disorders of the nervous system, use of imaging to the understanding of brain pathology and brain abnormalities, cognition and aging, social neuroscience, sensorimotor processing, communication and learning.
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