Differentiation Between Pure Cultures of Streptococcus pyogenes and Pseudomonas aeruginosa by FT-ICR-MS Volatile Analysis

Abstract

Two common bacteria, Streptococcus pyogenes and Pseudomonas aeruginosa, were differentiated based on their volatile metabolic waste products. The bacteria were cultured in a closed system and the headspace above the culture medium were collected, preconcentrated, and analyzed using a gas chromatography Fourier transform ion cyclotron reso- nance mass spectrometer (GC/FT-ICR MS).