Synthesis of Hydroxyapatite/Ag/TiO2 Nanotubes and Evaluation of Their Anticancer Activity on Breast Cancer Cell Line MCF-7

Abstract

In this research, TiO 2 nanotubes were synthesized by anodized oxidation method and were covered with a hydroxyapatite-silver nanoparticles using photodeposition and dip coating for loading silver nanoparticles and coated hydroxyapatite (HA). The morphological texture of TiO 2 nanotube and Ag-HA nanoparticles on TiO 2 nanotubes surface were studied by field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDAX analysis) and X-ray diffraction (XRD). The MCF-7 cell lines were treated with concentrations 1, 10 and 100 µg/ml of TiO 2 nanotubes and HA/Ag/TiO 2 nanotube for 24 and 48h. Finally, the cell viability and IC50% were evaluated using MTT assay. The results show that the HA/Ag/TiO 2 has more positive effect on enhancing the cell death compare to TiO 2 nanotubes and also exerts a time and concentration-dependent inhibition effect on viability of MCF-7 cells.