A search for anti-carcinogenic and cytotoxic effects of Persian Gulf sea snake (Enhydrina schistosa) venom on hepatocellular carcinoma using mitochondria isolated from liver

Q4 Pharmacology, Toxicology and Pharmaceutics

Iranian Journal of Pharmaceutical Sciences Pub Date : 2020-01-01 DOI:10.22034/IJPS.2019.109695.1572

Enayatollah Seydi, Pardis Tajarri, P. Naserzadeh, Jalal Pourahmad Jaktaji

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Abstract

Common techniques for the treatment of Hepatocellular carcinoma (HCC) have not been successful, and thus the design and discovery of new compounds with better anti-cancer function are needed. Snake venom is among the most important compounds used by researchers to the treatment of various cancers. This study was designed to evaluate the toxicity effect of Persian Gulf snake venom (Enhydrina schistosa) on hepatocytes and mitochondria isolated from HCC rats model. HCC has been induced in rats with diethylnitrosamine (DEN) and 2-acetylaminofluorene (2-AAF). Then rat hepatocytes were isolated with collagen perfusion technique. The results showed that E. schistosa (5, 10, 20 and 40 µg/ml) increases the level of reactive oxygen species (ROS) generation, collapse in mitochondrial membrane potential (MMP), swelling in mitochondria, and cytochrome c release only in hepatocytes and mitochondria isolated from the HCC group. These results proposed that E. schistosa could be considered as a promising complementary therapeutic agent for the treatment of HCC.

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利用肝分离线粒体研究波斯湾海蛇(Enhydrina schistosa)毒液对肝癌的抗癌和细胞毒作用

治疗肝细胞癌(HCC)的常用技术尚未取得成功，因此需要设计和发现具有更好抗癌功能的新化合物。蛇毒是研究人员用来治疗各种癌症的最重要的化合物之一。本研究旨在评价波斯湾蛇毒(Enhydrina schistosa)对肝细胞癌模型大鼠肝细胞和线粒体的毒性作用。用二乙基亚硝胺(DEN)和2-乙酰氨基芴(2-AAF)诱导大鼠肝细胞癌。采用胶原灌注法分离大鼠肝细胞。结果表明，血吸虫菌(5、10、20和40µg/ml)仅在肝细胞和HCC组分离的线粒体中增加活性氧(ROS)生成水平、线粒体膜电位(MMP)崩溃、线粒体肿胀和细胞色素c释放。上述结果提示血吸虫可作为HCC治疗的一种有前景的补充治疗剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.50

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0.00%

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期刊介绍： Iranian Journal of Pharmaceutical Sciences (IJPS) is an open access, internationally peer-reviewed journal that seeks to publish research articles in different pharmaceutical sciences subdivisions: pharmacology and toxicology, nanotechnology, pharmaceutics, natural products, biotechnology, pharmaceutical chemistry, clinical pharmacy and other pharmacy related topics. Each issue of the journal contents 16 outstanding research articles in area of pharmaceutical sciences plus an editorial written by the IJPS editors on one of the most up to date advances topics in pharmacy. All articles published by IJPS would be permanently accessible online freely without any subscription charges. Authors of the published articles have granted the right to use and disseminate their article to third parties.