Effect of plant growth regulators on growth patterns and enzymatic antioxidant activities in Hypericum calycinum shoot cultures

IF 0.5 Q4 ENVIRONMENTAL SCIENCES
G. Treneva, Y. Markovska, E. Wolfram, K. Danova
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引用次数: 4

Abstract

TRENEVA, G., Y. MARKOVSKA, E. WOLFRAM and K. DANOVA, 2014. Effect of plant growth regulators on growth patterns and enzymatic antioxidant activities in Hypericum calycinum shoot cultures. Bulg. J. Agric. Sci., Supplement 1: 46–50 Hypericum calycinum L. (section Ascyreia) is a species of the Hypericum genus, relative to the medicinal plant H. perforatum (St. Jonn’s worth). Unlike the widely studied H. perforatum, it is characterized by the lack of hypericins production. However, research has revealed its commensurable antidepressant activity, as compared with the latter species, and shown the presence of polyphenolics with marked radical scavenging activity. In order to develop an in vitro system with the potential of a novel source of bioactive constituent’s characteristic for the Hypericum genus, we initiated in vitro cultures of H. calycinum. In the present work we studiy the effect of exogenous BA and IBA treatments on the developmental patterns, enzymatic and non-enzymatic antioxidant defence of hypericin non-producing Hypericum calycinum shoot cultures. It was shown that supplementation of PGR led to stimulation of phenylalanine ammonia-lyase and superoxide dismuthase, as well as non-enzymatic antioxidant glutathione. However, they inhibited glutathione reductase, as well as lowered the levels of fl avonoids, ascorbate and dehydroascorbate in comparison with plant growth regulators-free control. Further on, it was established that elevation of IBA concentration slightly stimulated axillary shoot formation and shoot length, but inhibited polyphenolic levels in vitro. These results are in agreement with our previous results of interrelations between biomass formation and polyphenolics production in other Hypericum species in vitro. An in vitro culture system optimization is in progress in order to increase biomass production and retain biosynthetic capacity of the species. Abbreviations: BA – benzyl adenine, IBA – indole-3-butyric acid, PGR – plant growth regulators, DPPH 2,2-diphenyl-1-picrylhydrazyl, NO – nitric oxide, BAS – Bulgarian Academy of Sciences, PAL – Phenylalanine ammonia-lyase, SOD – superoxide dismuthase, GR – glutathione reductase, APX – ascorbate peroxidase, CAT – catalase, AsA – ascorbate, DHA – dehydrosacorbate, GSSG – oxidized glutathione, GSH – reduced glutathione, NADPH – Reduced nicotinamide adenine dinucleotide phosphate, KH 2 PO 4 – potassium phosphate monobasic, MgCl 2 – magnesium chloride, EDTA – Ethylenediaminetetraacetic acid, NaAA – sodium ascorbate, H 2 O 2 – hydrogen peroxide, DTNB – 5,50-dithiobis – (2-nitrobenzoic acid).
植物生长调节剂对萼花金丝桃生长模式及酶促抗氧化活性的影响
陈志强,陈志强,陈志强,陈志强,2014。植物生长调节剂对萼花金丝桃生长模式及酶促抗氧化活性的影响。Bulg。j·阿格利司。科学。金丝桃属(Hypericum calycinum L.,增刊1:46-50)是金丝桃属的一种,相对于药用植物贯叶连翘(St. Jonn 's worth)。与广泛研究的贯叶连翘不同,它的特点是缺乏金丝桃素的产生。然而,研究表明,与后者相比,其抗抑郁活性相当,并表明其多酚类物质具有显著的自由基清除活性。为了开发一种具有金丝桃属生物活性成分新来源潜力的离体体系,我们开始了金丝桃属植物的离体培养。本文研究了外源BA和IBA处理对不产金丝桃素的金丝桃芽培养物发育模式、酶促和非酶促抗氧化防御的影响。结果表明,添加PGR可刺激苯丙氨酸解氨酶和超氧化物二甲基化酶以及非酶抗氧化剂谷胱甘肽。然而,与不含植物生长调节剂的对照相比,它们抑制了谷胱甘肽还原酶,并降低了类黄酮、抗坏血酸和脱氢抗坏血酸的水平。进一步证实,IBA浓度的升高轻微刺激了腋芽的形成和芽长,但抑制了体外多酚水平。这些结果与我们之前对其他金丝桃属植物的生物量形成与多酚物质产生的相互关系的研究结果一致。为了提高生物量产量和保持该物种的生物合成能力,正在对体外培养系统进行优化。缩写:BA -苄基腺嘌呤、IBA -吲哚-3-丁酸、PGR -植物生长调节剂、DPPH 2、2-二苯基-1-苦酰肼、NO -一氧化氮、BAS -保加利亚科学院、PAL -苯丙氨酸解氨酶、SOD -超氧化物二甲基化酶、GR -谷胱甘肽还原酶、APX -抗坏血酸过氧化物酶、CAT -过氧化氢酶、AsA -抗坏血酸、DHA -脱氢甘油三酯、GSSG -氧化谷胱甘肽、GSH -还原谷胱甘肽、NADPH -还原烟酰胺腺嘌呤二核苷酸磷酸、khpo4 -磷酸氢钾,mgcl2 -氯化镁,EDTA -乙二胺四乙酸,NaAA -抗坏血酸钠,h2o2 -过氧化氢,DTNB - 5,50-二硫比斯-(2-硝基苯甲酸)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Bulgarian Journal of Agricultural Science
Bulgarian Journal of Agricultural Science Veterinary-General Veterinary
CiteScore
1.00
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Bulgarian Journal of Agricultural Science (BJAS) is a peer-reviewed, open access, online and print journal, issued bimonthly. It has been published in English since 1995. It publishes original research papers, review articles and brief communications from all areas of agricultural science including agricultural economics, agroecology, agrochemisty and soil science, crop science, plant protection, animal science, veterinary medicine, fisheries and aquaculture, agricultural engineering, food technologies and other related areas.
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