Protein to polysaccharide ratio in EPS as an indicator of non-optimized operation of tertiary nitrifying MBBR

IF 2 Q3 Environmental Science

Water Quality Research Journal of Canada Pub Date : 2016-11-02 DOI:10.2166/WQRJC.2016.040

Baisha Ren, B. Young, F. Variola, R. Delatolla

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引用次数: 13

Abstract

The protein (PN), polysaccharide (PS), and extracellular DNA (eDNA) percent concentrations of extracellular polymeric substances (EPS) of biofilms samples harvested from a pilot-scale nitrifying moving bed biofilm reactor (MBBR) were investigated at various operating temperatures and hydraulic retention times (HRTs). Chemically measured EPS PN/PS ratios were shown to correlate to Raman intensity ratios of amide III to carbohydrate at 362 rel. cm−1. The study also demonstrates that tertiary nitrifying MBBR systems may be optimized to operate at HRTs as low as 0.75 to 1.0 h as opposed to conventional HRTs of 2.0 to 6.0 h. The EPS of the nitrifying MBBR biofilm exhibited the lowest percent PN content and the highest percent PSs and eDNA content. In particular, the PN/PS ratios lower than 3 were indicative of non-optimal operation of the nitrifying MBBR systems; whereas PN/PS ratios with values significantly below 3 were observed for ammonia underloaded systems at high operating temperatures and hydraulically overloaded systems at low HRTs. This study demonstrates that the PN/PS ratio in EPS is a potential metric to identify non-optimal operation of nitrifying MBBR systems.

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EPS蛋白多糖比作为三级硝化MBBR非优化运行的指标

在不同的操作温度和水力保留时间(HRTs)下，研究了从中试规模的硝化移动床生物膜反应器(MBBR)中收获的生物膜样品的细胞外聚合物质(EPS)的蛋白质(PN)、多糖(PS)和细胞外DNA (eDNA)百分比浓度。化学测量的EPS PN/PS比率与酰胺III与碳水化合物在362 rel. cm−1时的拉曼强度比相关。研究还表明，三级硝化MBBR系统可以优化为在0.75 ~ 1.0 h的hrt下运行，而不是传统的2.0 ~ 6.0 h。硝化MBBR生物膜的EPS表现出最低的PN含量，最高的ps和eDNA含量。特别是，PN/PS低于3表明硝化MBBR系统运行不优化;然而，在高工作温度下氨负荷不足的系统和低hrt下水力过载的系统中，PN/PS值明显低于3。该研究表明，EPS中的PN/PS比率是识别硝化MBBR系统非最佳操作的潜在指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Water Quality Research Journal of Canada WATER RESOURCES-

CiteScore

1.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Water Quality Research Journal publishes peer-reviewed, scholarly articles on the following general subject areas: Impact of current and emerging contaminants on aquatic ecosystems Aquatic ecology (ecohydrology and ecohydraulics, invasive species, biodiversity, and aquatic species at risk) Conservation and protection of aquatic environments Responsible resource development and water quality (mining, forestry, hydropower, oil and gas) Drinking water, wastewater and stormwater treatment technologies and strategies Impacts and solutions of diffuse pollution (urban and agricultural run-off) on water quality Industrial water quality Used water: Reuse and resource recovery Groundwater quality (management, remediation, fracking, legacy contaminants) Assessment of surface and subsurface water quality Regulations, economics, strategies and policies related to water quality Social science issues in relation to water quality Water quality in remote areas Water quality in cold climates The Water Quality Research Journal is a quarterly publication. It is a forum for original research dealing with the aquatic environment, and should report new and significant findings that advance the understanding of the field. Critical review articles are especially encouraged.