Results of RT-PCR test for the presence of SARS-CoV-2 in multiple organs of post-COVID patients who died from causes other than COVID-19

Q4 Medicine
S. Petrikov, M. Godkov, A. Kanibolotsky, N.S. Dyakun, A. Shabanov, V. Kulabukhov, A. Leinsoo, A. Bazhenov
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引用次数: 1

Abstract

The aim of this research work was to study the ability of SARS-CoV-2 to persist in multiple organs of people who have recovered from COVID-19. For this purpose, autopsy material was used from patients with a history of COVID-19 who died from causes unrelated to a new coronavirus infection. Autopsy material collected from 11 different organs was analyzed for the presence of SARS-CoV-2 RNA using real-time polymerase chain reaction (RT-PCR). Among the organs tested, SARS-CoV-2 RNA persisted longest in the bronchi and lungs. Thus, in both patients tested positive more than 100 days after recovery, SARS-CoV-2 RNA was detected only in the bronchi and lungs. On the contrary, the shorter the recovery time was, the more organs were found to have SARS-Cov-2 RNA. SARS-CoV-2 RNA was most frequently detected in biomaterials of deceased people over 80 years of age, which may indicate a longer period of virus elimination in the elderly. No correlation between the distribution of SARS-CoV-2 across organs and the age of patients could be detected, but it was shown that SARS-CoV-2 persisted in more organs in males after recovery. In females, SARS-CoV-2 RNA was found predominantly in the bronchi and lungs. Key words: SARS-CoV-2, autopsy material, real-time polymerase chain reaction
非死因死亡患者多器官SARS-CoV-2的RT-PCR检测结果
这项研究工作的目的是研究SARS-CoV-2在COVID-19康复者的多个器官中持续存在的能力。为此,研究人员使用了有COVID-19病史的患者的尸检材料,这些患者死于与新型冠状病毒感染无关的原因。利用实时聚合酶链反应(RT-PCR)分析从11个不同器官收集的尸检材料是否存在SARS-CoV-2 RNA。在检测的器官中,SARS-CoV-2 RNA在支气管和肺部持续时间最长。因此,在康复后100多天检测呈阳性的两名患者中,仅在支气管和肺部检测到SARS-CoV-2 RNA。相反,恢复时间越短,携带SARS-Cov-2 RNA的器官越多。SARS-CoV-2 RNA最常见于80岁以上死者的生物材料中,这可能表明老年人的病毒消除期较长。SARS-CoV-2在各器官的分布与患者的年龄没有相关性,但研究表明,在男性康复后,SARS-CoV-2在更多器官中持续存在。在女性中,SARS-CoV-2 RNA主要存在于支气管和肺部。关键词:SARS-CoV-2,尸检材料,实时聚合酶链反应
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来源期刊
Infektsionnye Bolezni
Infektsionnye Bolezni Medicine-Infectious Diseases
CiteScore
1.30
自引率
0.00%
发文量
15
期刊介绍: The journal publishes original research works, reviews of literature, lectures, methodological recommendations, clinical observations. Main topics: problems of etiology, pathogenesis, clinical manifestations of infectious diseases, new techniques and methods of their diagnosis, prevention and treatment; special attention is paid to the problems of antibacterial and antiviral therapy, the use of immunoglobulins and interferons, and also to intensive therapy of critical states. The journal is in the List of leading scientific journals and periodicals of the Supreme Attestation Committee, where the principal results of doctoral dissertations should be published.
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