Circulation of influenza viruses in the epidemic season of 2018–2019 among people residing in Northern and Western Kazakhstan

Q4 Medicine

Infektsionnye Bolezni Pub Date : 2021-01-01 DOI:10.20953/1729-9225-2021-2-70-75

T. Glebova, Almaty Kazakhstan Virology», N. Klivleyeva, A. Baimukhametova, N. Saktaganov, G. Lukmanova, N. Ongarbayeva, M. Shamenova, B. Baimakhanova

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Abstract

Objective. Detection of influenza viruses among the population on the territory of the Northern and Western Kazakhstan during the 2018–2019 epidemic season. Patients and methods. The study involved 835 patients with ARVI symptoms. Biological samples were screened in real-time polymerase chain reaction (RT-PCR), hemagglutination inhibition (HAI) assay, and enzyme-linked immunosorbent assay (ELISA). Hemagglutinating agents were isolated in 9-10-day-old developing chicken embryos. Identification of isolates was carried out in RT-PCR and HAI assay. Results. 936 clinical samples (835 nasopharyngeal swabs and 101 blood serums) were collected from patients in the Northern (North Kazakhstan and Pavlodar oblasts) and Western (West Kazakhstan oblast) regions during the 2018–2019 epidemic season. Primary screening of 835 nasopharyngeal swabs revealed the genetic material of influenza virus in 20.48%, influenza A virus in 20.36%, and influenza B virus in 0.12%. Subtyping of PCR positive samples for influenza type A virus showed the presence of the genetic material of influenza A/H1N1pdm09 virus in 14.01%, A/H3N2 virus in 4.91%. The virus subtype was not established in 1.66%. Virological examination of nasopharyngeal swabs led to obtaining 14 isolates, of which 13 were identified as influenza A/H1N1pdm09 viruses and 1 as influenza A/H3N2 virus. Serological studies of 101 blood serums in the HAI assay showed the presence of antihemagglutinins against influenza A/H1N1pdm09 virus in 28.71%, A/H3N2 virus in 30.69%, type B virus in 3.96% of the total number of samples. Antibodies simultaneously against two subtypes of influenza viruses (A and B) were detected in 12.87% of cases. In ELISA antibodies against influenza A/H1N1 virus were revealed in 24.75% of cases, A/H3N2 virus in 19.80%, type B virus in 14.85%. Antibodies simultaneously against two types of influenza viruses (A and B) were detected in 2.97% of blood serums. Conclusion. The results of virological and serological studies presented in the paper suggest circulation of influenza A/H1N1pdm09, A/H3N2, and type B viruses in the examined oblasts of Kazakhstan during the 2018–2019 season. Key words: virus, hemagglutinin, influenza, diagnosis, isolate, neuraminidase, circulation

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2018-2019年流行季节居住在哈萨克斯坦北部和西部人群中的流感病毒传播情况

目标。2018-2019年流行季节期间哈萨克斯坦北部和西部地区人口中流感病毒的检测情况。患者和方法。该研究涉及835名有ARVI症状的患者。采用实时聚合酶链反应(RT-PCR)、血凝抑制(HAI)和酶联免疫吸附试验(ELISA)筛选生物样品。从9 ~ 10日龄发育中的鸡胚中分离到血凝剂。采用RT-PCR和HAI法对分离株进行鉴定。结果:在2018-2019年流行季，从北部(北哈萨克斯坦和巴甫洛达尔州)和西部(西哈萨克斯坦州)地区的患者中收集了936份临床样本(835份鼻咽拭子和101份血清)。对835份鼻咽拭子进行初步筛查，发现流感病毒遗传物质占20.48%，甲型流感病毒占20.36%，乙型流感病毒占0.12%。甲型流感病毒PCR阳性标本分型结果显示，甲型h1n1 / pdm09流感病毒遗传物质占14.01%，甲型H3N2流感病毒遗传物质占4.91%。1.66%未确定病毒亚型。经鼻咽拭子病毒学检查，获得14株分离株，其中13株为甲型流感/H1N1pdm09病毒，1株为甲型流感/H3N2病毒。经血清学分析，101份血清中存在抗流感病毒A/H1N1pdm09的血凝素，占总样本数的28.71%，A/H3N2占30.69%，B型占3.96%。12.87%的病例同时检测到两种流感病毒亚型(A和B)的抗体。ELISA检测发现甲型H1N1流感病毒抗体占24.75%，甲型H3N2流感病毒抗体占19.80%，乙型流感病毒抗体占14.85%。2.97%的患者血清中同时检测到A、B两种流感病毒抗体。结论。本文中提出的病毒学和血清学研究结果表明，2018-2019年期间，在哈萨克斯坦所检查的州中存在甲型h1n1流感病毒、甲型H3N2流感病毒和乙型流感病毒的传播。关键词:病毒，血凝素，流感，诊断，分离，神经氨酸酶，循环

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来源期刊

Infektsionnye Bolezni Medicine-Infectious Diseases

CiteScore

1.30

自引率

0.00%

发文量

期刊介绍： The journal publishes original research works, reviews of literature, lectures, methodological recommendations, clinical observations. Main topics: problems of etiology, pathogenesis, clinical manifestations of infectious diseases, new techniques and methods of their diagnosis, prevention and treatment; special attention is paid to the problems of antibacterial and antiviral therapy, the use of immunoglobulins and interferons, and also to intensive therapy of critical states. The journal is in the List of leading scientific journals and periodicals of the Supreme Attestation Committee, where the principal results of doctoral dissertations should be published.