First report of Xanthomonas citri Subsp. Citri causing citrus canker on grape fruit (Citrus paradisi), washington naval (Citrus sinensis), kaghzi limon (Citrus aurantifolia Swingle), lemon (Citrus lim

IF 0.7 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Pakistan Journal of Agricultural Sciences Pub Date : 2021-09-01 DOI:10.21162/pakjas/21.9701

Muhammad Shah Nawaz ul Rehman

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引用次数: 0

Abstract

Citrus fruit production is largely affected by different bacterial and fungal pathogens. In Pakistan bacterial diseases like citrus bacterial canker (CBC) pose severe risk to citrus economy. Diagnoses of such diseases could be helpful to avoid the epidemics in nurseries or orchids. In 2011-12, citrus canker symptoms i.e., callus-like outgrowths on leaves and fruits of grape fruit (Citrus paradisi), Washington naval (Citrus sinensis), Kaghzi Limon (Citrus aurantifolia swingle), lemon (Citrus Limon) and pomelo (Citrus maxima) were noticed in Sargodha district of Punjab, Pakistan. Bacteria i.e., yellow mucoid, Xanthomonas- like isolates, were isolated from these lesions. Bacteria isolated from these lesions were cultured and total DNA was isolated. A diagnostic fragment of 581 bp based on rpf genes of Xanthomonas citri pv. citri was amplified, cloned and completely sequenced. BLAST and evolutionary analysis revealed that these isolates show 100% sequence similarity and group with Xanthomonas citri subsp. citri from Argentina (CP023285) and Reunion (CP018858), (CP018854). To our knowledge, this is the first formal report of X. campestris pv. citri pathotypes A on Citrus paradise, Citrus sinensis, Citrus maxima, Citrus Limon and Citrus aurantifolia swingle in Pakistan

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柑橘黄单胞菌亚属首次报道。引起柑橘溃疡病的柑橘属植物有葡萄(citrus paradisi)，华盛顿海军(citrus sinensis)，卡格齐柠檬(citrus aurantifolia Swingle)，柠檬(citrus lim)

柑橘类水果的生产在很大程度上受到不同细菌和真菌病原体的影响。在巴基斯坦，柑橘细菌性溃疡病(CBC)等细菌性疾病对柑橘经济构成严重威胁。这些疾病的诊断有助于避免苗圃或兰花的流行。2011- 2012年，在巴基斯坦旁遮普省Sargodha地区发现了柑橘溃疡病症状，即葡萄(citrus paradisi)、华盛顿(citrus sinensis)、Kaghzi Limon (citrus aurantifolia swingle)、柠檬(citrus Limon)和柚子(citrus maxima)的叶子和果实上出现了类似愈伤组织的生长。从这些病变中分离出黄单胞菌样细菌，即黄色黏液。培养从这些病变中分离的细菌并分离总DNA。基于柑橘黄单胞菌rpf基因的581 bp诊断片段。对Citri进行了扩增、克隆和完全测序。BLAST和进化分析表明，这些分离株序列与柑橘黄单胞菌亚群具有100%的相似性和类群。产自阿根廷(CP023285)和留尼汪岛(CP018858) (CP018854)的柑橘。据我们所知，这是X. campestris pv的第一次正式报告。柑桔病型A在巴基斯坦的天堂柑桔、中华柑桔、大黄柑桔、柠檬柑桔和金叶柑桔上的分布

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来源期刊

Pakistan Journal of Agricultural Sciences AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

1.80

自引率

25.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Pakistan Journal of Agricultural Sciences is published in English four times a year. The journal publishes original articles on all aspects of agriculture and allied fields.