CORRELATION OF 16S RRNA WITH SERUM LEVELS OF THE CYTOKINES, TNF-α AND IL-1β, IN SUBJECTS WITH A POSITIVE HELICOBACTER PYLORI STOOL ANTIGEN TEST (HPSA)

Ayat Moradipour, A. Khosravi, M. Mehrabi, S. Faryadian
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引用次数: 5

Abstract

Introduction: Helicobacter pylori (H. pylori), is a bacterium responsible for upper gastrointestinal tract diseases. The 16s rRNA is a common H. pylori gene which are usually preferred for diagnosis purpose. The aim of this study was to determine the prevalence and abundance of 16s rRNA in fecal samples and also evaluate correlation between the level of 16s rRNA and activities of the cytokines, TNF-α and IL-1β, in serum. Materials and methods: The present study was performed on 84 subjects with digestion problems. Fecal and blood samples were collected and 16s rRNA gene was assayed using PCR. The serum levels of TNF-α and IL-1β levels were measured by enzyme-linked immunosorbent assay (ELISA). Results: The results of the study revealed that there was a positive correlation between the 16s rRNA gene, H. pylori stool antigen (HPSA) and TNF-α cytokine. The study also noted that with every unit of increase in either of the quantified parameters of HPSA and IL-1β, the presence of 16s rRNA in fecal samples, showed a 2.98 and 1.01 times rise, respectively. Conclusion: According to the obtained results, it may be concluded that activities of cytokine TNF-a correlated well with the presence of HPSA and 16srRNA gene in the stomach’s lining. Increase in the activities of HPSA and TNF-a cytokine could be associated with the presence of 16s rRNA in feces.
幽门螺杆菌粪便抗原检测(hpsa)阳性受试者血清细胞因子、tnf -α和il-1β水平与16s rrna的相关性研究
简介:幽门螺杆菌(h.p ylori)是一种导致上消化道疾病的细菌。16s rRNA是一种常见的幽门螺杆菌基因,通常用于诊断目的。本研究的目的是确定粪便样本中16s rRNA的流行度和丰度,并评估16s rRNA水平与血清中细胞因子TNF-α和IL-1β活性的相关性。材料与方法:本研究对84例有消化问题的受试者进行了研究。采集粪便和血液标本,采用PCR法检测16s rRNA基因。采用酶联免疫吸附法(ELISA)检测血清TNF-α和IL-1β水平。结果:本研究结果显示16s rRNA基因、幽门螺杆菌粪便抗原(HPSA)与TNF-α细胞因子呈正相关。该研究还指出,HPSA和IL-1β的量化参数每增加一个单位,粪便样品中16s rRNA的存在分别增加2.98倍和1.01倍。结论:根据所获得的结果,可以得出细胞因子TNF-a的活性与胃粘膜HPSA和16srRNA基因的存在有良好的相关性。HPSA和TNF-a细胞因子活性的升高可能与粪便中16s rRNA的存在有关。
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