{"title":"Resveratrol Attenuates Heat-Stress-Impaired Immune and Inflammatory Responses of Broilers by Modulating Toll-Like Receptor-4 Signaling Pathway","authors":"Z. Li, A. Liu, J. Xu, C. Zhang","doi":"10.1590/1806-9061-2022-1668","DOIUrl":null,"url":null,"abstract":"This study investigated the effect of resveratrol on the immune and inflammatory responses and the mRNA levels of splenic toll-like receptor (TLR)-4 signaling pathway-related genes of broilers under heat stress (HS). One hundred and sixty-two birds were allocated to three groups, each with 6 replicates, for 21 continuous days. The three treatments were as follows: the control group (22 ± 1 °C), the HS (33 ± 1 °C for 10 h d -1 and 22 ± 1 °C for the remaining time) group and the HS + resveratrol (400 mg kg -1 ) group. At the end of the trial, one bird per replicate close to the average body weight (BW) was selected, exsanguinated, and slaughtered. Compared with the control group, the HS treatment decreased ( p <0.05) final BW, average daily gain (ADG), average daily feed intake (ADFI), relative weight of bursa of Fabricius and spleen, serum immunoglobulin (Ig) Y, IgA and interleukin (IL)-10 contents, and splenic IL-10 mRNA level, while it increased ( p <0.05) feed/gain, mRNA levels of splenic tumor necrosis factor-α (TNF-α ), TLR-4, nuclear factor-kappa-B (NF-κ B), IL-1 β , and IL-6. Compared to the HS group, the HS+resveratrol group exhibited increased ( p <0.05) final BW, ADG, relative weight of bursa of Fabricius and spleen, serum IgY, IgA and IL-10 contents, and splenic IL-10 mRNA level, while it exhibited lower ( p <0.05) TNF-α , IL-1 β and IL-6 contents in serum, and splenic TLR4, TNF-α , IL-1 β , and NF-κ B mRNA levels. In conclusion, resveratrol prevented a HS-impairment of the immune function of broilers by blocking the abnormal activation of the TLR4 signaling pathway.","PeriodicalId":9244,"journal":{"name":"Brazilian Journal of Poultry Science","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Brazilian Journal of Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1590/1806-9061-2022-1668","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
This study investigated the effect of resveratrol on the immune and inflammatory responses and the mRNA levels of splenic toll-like receptor (TLR)-4 signaling pathway-related genes of broilers under heat stress (HS). One hundred and sixty-two birds were allocated to three groups, each with 6 replicates, for 21 continuous days. The three treatments were as follows: the control group (22 ± 1 °C), the HS (33 ± 1 °C for 10 h d -1 and 22 ± 1 °C for the remaining time) group and the HS + resveratrol (400 mg kg -1 ) group. At the end of the trial, one bird per replicate close to the average body weight (BW) was selected, exsanguinated, and slaughtered. Compared with the control group, the HS treatment decreased ( p <0.05) final BW, average daily gain (ADG), average daily feed intake (ADFI), relative weight of bursa of Fabricius and spleen, serum immunoglobulin (Ig) Y, IgA and interleukin (IL)-10 contents, and splenic IL-10 mRNA level, while it increased ( p <0.05) feed/gain, mRNA levels of splenic tumor necrosis factor-α (TNF-α ), TLR-4, nuclear factor-kappa-B (NF-κ B), IL-1 β , and IL-6. Compared to the HS group, the HS+resveratrol group exhibited increased ( p <0.05) final BW, ADG, relative weight of bursa of Fabricius and spleen, serum IgY, IgA and IL-10 contents, and splenic IL-10 mRNA level, while it exhibited lower ( p <0.05) TNF-α , IL-1 β and IL-6 contents in serum, and splenic TLR4, TNF-α , IL-1 β , and NF-κ B mRNA levels. In conclusion, resveratrol prevented a HS-impairment of the immune function of broilers by blocking the abnormal activation of the TLR4 signaling pathway.
期刊介绍:
A Revista Brasileira de Ciência Avícola surgiu em 1999 a partir da necessidade que a comunidade científica possuía de um periódico para veiculação e publicação de seus trabalhos, com a publicação de três números anuais.
A Revista conta hoje com um corpo editorial altamente qualificado e com artigos científicos desenvolvidos pelos maiores especialistas da área, o que a cada dia atrai mais leitores em busca de inovação e respaldo técnico.
Devido à credibilidade que conquistou pelos esforços de sus autores, relatores e revisores, a Revista ganhou caráter de coleção, sendo consultada como fonte segura de estudo desenvolvidos na Avicultura.
A partir de 2003 – volume 5 -, a Revista passou a chamar-se Brazilian Journal of Poultry Science, e todos os trabalhos passaram a ser publicados em inglês. No mesmo ano subiu para quatro o número de revistas por volume, ampliando-se assim os trabalhos publicados anualmente.