Smartphone-based rapid and low-cost method for the determination of eugenol content of clove essential oil

IF 0.8 4区农林科学 Q3 AGRONOMY

Ciencia Rural Pub Date : 2023-04-14 DOI:10.1590/0103-8478cr20220498

Izaura Ceolin dos Santos, S. K. Schlesner, D. P. Moraes, Daniele de Freitas Fereira, M. Voss, S. R. Giacomelli, J. Barin

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引用次数: 0

Abstract

ABSTRACT: The determination of eugenol in clove essential oil was performed using a smartphone-operated device, which was used for image capture and processing. The colorimetric reaction with the Folin-Ciocalteu reagent was used, and the lighting conditions were evaluated to capture images directly in 2 mL disposable vessels. The free application PhotoMetrix UVC was used for partial least squares regression calibration with suitable values (R² greater than 0.99). The accuracy of the proposed method was compared with traditional methods, such as gas chromatography (GC) and spectrophotometry, and it can be observed that there were no significant differences (Student’s t-test (P > 0.05), with agreements from 97% to 101%. The smartphone method allowed the evaluation of several samples in a few minutes, with simple analysis steps and easy interpretation of the results. The miniaturized scale allowed the use of small amounts of reagents with minimal waste generation. Therefore, the proposed method can be easily operated in the field and allows the evaluation of the quality of clove essential oil in places of restricted access without the need for a laboratory structure and specialized analysts.

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基于智能手机的丁香精油中丁香酚含量的快速低成本测定方法

摘要:采用智能手机操作仪对丁香精油中丁香酚的含量进行测定，并进行图像采集和处理。使用Folin-Ciocalteu试剂进行比色反应，并评估光照条件，直接在2ml一次性容器中捕获图像。利用免费应用PhotoMetrix UVC进行偏最小二乘回归校正，得到合适的值(R²大于0.99)。将该方法与传统的气相色谱(GC)、分光光度法等方法的准确度进行了比较，发现两者之间没有显著差异(学生t检验(P < 0.05)，一致性在97% ~ 101%之间。智能手机方法可以在几分钟内评估几个样本，分析步骤简单，结果易于解释。小型化的规模允许使用少量的试剂，产生最少的废物。因此，所提出的方法可以很容易地在现场操作，并且允许在限制进入的地方评估丁香精油的质量，而不需要实验室结构和专业分析人员。

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来源期刊

Ciencia Rural AGRONOMY-

CiteScore

1.70

自引率

0.00%

发文量

233

审稿时长

2-4 weeks

期刊介绍： The purpose of Ciência Rural is to publish the results of original research, note and reviews which contribute significantly to knowledge in Agricultural Sciences. Preference will be given to original articles that develop news concepts or experimental approaches and are not merely repositories of scientific data. The decison of acceptance for publication lies with the Editors and is based on the recommendations of Editorial Comission, Area Committee and/ or ad hoc reviewers. The editors and reviewers are external to the institution.