Protocol for in vitro rooting ofPyruscomunnisrootstocks

IF 0.8 4区农林科学 Q3 AGRONOMY

Ciencia Rural Pub Date : 2023-05-12 DOI:10.1590/0103-8478cr20210661

M. Nadal, Otávio Bernardes Machado, Ronilson Carlos de Araújo, F. A. Rodrigues, L. Rufato, J. Dória, M. Pasqual

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引用次数: 0

Abstract

ABSTRACT: Effective protocols for in vitro rooting for woody fruit trees are still a challenge for in vitro seedling production, especially when there is a need to insert new cultivars or rootstocks. These protocols are essential to accelerate studies in plant breeding programs and for seedling distribution. This study evaluated the use of 6-Benzylaminopurine (IBA) in in vitro rooting of Pyruscomunnis rootstocks, clones ‘OHxF87’ and Pyrodwarf. Explant exposure times (0, 24, 48, 72, and 96 hours) to 20 mg L-1 IBA were tested for in vitro rooting. The exposure to IBA resulted in rooting rates above 80%, surpassing some results reported in the literature. The 24-hour treatment provided 81,81% survival, leading to an average growth of five roots with 19 mm length, for ‘OHxF87’ rootstock. The same exposure time resulted in the highest survival rate (75%) and the highest mean root number, seven roots per plant with 10 mm length, for ‘PDW’ rootstock. Root formation did not occur in the absence of synthetic auxin. Therefore, it can be concluded that a 24-hour exposure at 20 mg L-1 IBA was sufficient to promote in vitro rooting in ‘OHxF87’ and Pyrodwarf rootstocks’.

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黄豆砧木离体生根的研究

摘要:木本果树离体生根的有效方案仍然是离体苗生产的一个挑战，特别是当需要插入新的品种或砧木时。这些协议对于加快植物育种计划和幼苗分配的研究至关重要。本研究评价了6-苄基氨基嘌呤(IBA)在Pyruscomunnis砧木、OHxF87无性系和Pyrodwarf无性系离体生根中的应用。对外植体暴露于20 mg L-1 IBA的时间(0、24、48、72和96小时)进行离体生根试验。暴露于IBA导致生根率超过80%，超过了文献报道的一些结果。24小时处理后，‘OHxF87’砧木的成活率为81,81%，平均生长5根，根长为19 mm。相同的暴露时间导致“PDW”砧木的最高成活率(75%)和最高的平均根数(每株10 mm长7根)。在没有合成生长素的情况下不发生根的形成。由此可见，24小时20 mg L-1 IBA处理足以促进‘OHxF87’和焦矮砧木的离体生根。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ciencia Rural AGRONOMY-

CiteScore

1.70

自引率

0.00%

发文量

233

审稿时长

2-4 weeks

期刊介绍： The purpose of Ciência Rural is to publish the results of original research, note and reviews which contribute significantly to knowledge in Agricultural Sciences. Preference will be given to original articles that develop news concepts or experimental approaches and are not merely repositories of scientific data. The decison of acceptance for publication lies with the Editors and is based on the recommendations of Editorial Comission, Area Committee and/ or ad hoc reviewers. The editors and reviewers are external to the institution.