Electrochemical Determination of Dapoxetine HCl in Biological Fluids Using Coated Wire Electrod

Abstract

Coated wire sensor for potentiometric determination of DAP (dapoxetine HCl) in pure form and in biological fluids based on DAP-TPB (dapoxetine-tetraphenyl borate) as the sensing element in the presence of DOP (dioctylphthalate) as the plasticizing solvent mediator was prepared. The best performance was obtained with a membrane composition of 10.0% (w/w) ion-pair, 45.0% DOP (w/w) and 45.0% PVC (w/w). The electrode showed a Nernstian response (with a slope of 58.70 mV decade) for the concentration range of 4.2 × 10-1.0 × 10 mol/L. It illustrates a relatively fast response time in the whole concentration range (~15 s) in a pH range of 3.0-7.5 . The selectivity coefficients were determined in relation to several inorganic and organic species. DAP is determined successfully in pure solutions and in biological fluids using the standard additions and potentiometric titrations methods.